Detailed information of TA system TA291569

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 87275..87701
• Replicon: plasmid 2
• Accession: NZ_LR890607
• Organism: Escherichia coli isolate MINF_8D-sc-2280460

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JMW69_RS23790
• Protein ID: WP_001312861.1
• Coordinates: 87275..87433 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	87477..87701 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMW69_RS23755	82480..82614	-	135	Protein_95	TraY domain-containing protein	-
JMW69_RS23760	82702..83379	-	678	WP_001348626.1	PAS domain-containing protein	-
JMW69_RS23765	83513..83896	-	384	WP_001151566.1	relaxosome protein TraM	-
JMW69_RS23770	84227..84829	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
JMW69_RS23775	85126..85947	-	822	WP_001234469.1	DUF945 domain-containing protein	-
JMW69_RS23780	86066..86353	-	288	WP_000107535.1	hypothetical protein	-
JMW69_RS23785	86378..86584	-	207	WP_000275859.1	hypothetical protein	-
JMW69_RS23790	87275..87433	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	87477..87701	-	225	NuclAT_0	-	Antitoxin
-	87477..87701	-	225	NuclAT_0	-	Antitoxin
-	87477..87701	-	225	NuclAT_0	-	Antitoxin
-	87477..87701	-	225	NuclAT_0	-	Antitoxin
JMW69_RS23795	87513..87701	+	189	WP_001299721.1	hypothetical protein	-
JMW69_RS23800	87713..88432	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
JMW69_RS23805	88429..88863	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
JMW69_RS23810	88918..90876	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
JMW69_RS23815	90942..91175	-	234	WP_000005990.1	DUF905 family protein	-
JMW69_RS23820	91238..91777	-	540	WP_000290841.1	single-stranded DNA-binding protein	-
JMW69_RS23825	92420..92674	-	255	WP_047605447.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / aadA5 / qacE / sul1 / mph(A) / erm(B)	senB	1..126618	126618

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T291569 WP_001312861.1 NZ_LR890607:c87433-87275 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT291569 NZ_LR890607:c87701-87477 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin