Detailed information of TA system TA291243

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66629..67055
• Replicon: plasmid 2
• Accession: NZ_LR890509
• Organism: Escherichia coli isolate MSB1_9I-sc-2280417

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JMW57_RS23300
• Protein ID: WP_001312861.1
• Coordinates: 66629..66787 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	66831..67055 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMW57_RS23270	62003..62692	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
JMW57_RS23275	62879..63262	-	384	WP_001151524.1	relaxosome protein TraM	-
JMW57_RS23280	63583..64185	+	603	WP_112030399.1	transglycosylase SLT domain-containing protein	-
JMW57_RS23285	64481..65302	-	822	WP_001234469.1	DUF945 domain-containing protein	-
JMW57_RS23290	65420..65707	-	288	WP_000107535.1	hypothetical protein	-
JMW57_RS23295	66179..66334	-	156	WP_201524621.1	hypothetical protein	-
JMW57_RS23300	66629..66787	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	66831..67055	-	225	NuclAT_0	-	Antitoxin
-	66831..67055	-	225	NuclAT_0	-	Antitoxin
-	66831..67055	-	225	NuclAT_0	-	Antitoxin
-	66831..67055	-	225	NuclAT_0	-	Antitoxin
JMW57_RS23305	66867..67055	+	189	WP_001299721.1	hypothetical protein	-
JMW57_RS23310	67067..67786	-	720	WP_112030397.1	plasmid SOS inhibition protein A	-
JMW57_RS23315	67783..68217	-	435	WP_032217500.1	conjugation system SOS inhibitor PsiB	-
JMW57_RS23320	68272..70230	-	1959	WP_112030396.1	ParB/RepB/Spo0J family partition protein	-
JMW57_RS23325	70295..70528	-	234	WP_000006030.1	DUF905 family protein	-
JMW57_RS23330	70590..71129	-	540	WP_009426937.1	single-stranded DNA-binding protein	-
JMW57_RS23335	71602..71757	-	156	WP_165369128.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..91228	91228

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T291243 WP_001312861.1 NZ_LR890509:c66787-66629 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT291243 NZ_LR890509:c67055-66831 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin