Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 69039..69775 Replicon plasmid 2
Accession NZ_LR890361
Organism Klebsiella pneumoniae isolate INF049-sc-2279950

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag JMW75_RS25885 Protein ID WP_003026803.1
Coordinates 69293..69775 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag JMW75_RS25880 Protein ID WP_003026799.1
Coordinates 69039..69305 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW75_RS25860 65531..65839 - 309 WP_017896554.1 hypothetical protein -
JMW75_RS25870 67443..68423 - 981 WP_020806188.1 IS5-like element ISKpn26 family transposase -
JMW75_RS25875 68492..68794 + 303 Protein_72 cell envelope integrity protein TolA -
JMW75_RS25880 69039..69305 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
JMW75_RS25885 69293..69775 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
JMW75_RS25890 69976..71379 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
JMW75_RS25895 71408..72040 - 633 WP_001567369.1 hypothetical protein -
JMW75_RS25900 72564..73568 - 1005 WP_000427620.1 IS110-like element IS4321 family transposase -
JMW75_RS25905 73647..74204 - 558 WP_003100847.1 recombinase family protein -
JMW75_RS25910 74198..74569 - 372 WP_003100853.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..233670 233670
- inside IScluster/Tn - - 65946..80889 14943


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T290779 WP_003026803.1 NZ_LR890361:69293-69775 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT290779 WP_003026799.1 NZ_LR890361:69039-69305 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References