Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 25065..25708 Replicon plasmid 2
Accession NZ_LR890361
Organism Klebsiella pneumoniae isolate INF049-sc-2279950

Toxin (Protein)


Gene name vagD Uniprot ID A0A3Q8D6L7
Locus tag JMW75_RS25660 Protein ID WP_020804312.1
Coordinates 25292..25708 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag JMW75_RS25655 Protein ID WP_001261276.1
Coordinates 25065..25295 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW75_RS25615 20605..20718 + 114 WP_014343462.1 hypothetical protein -
JMW75_RS25620 20847..21104 + 258 WP_009310077.1 hypothetical protein -
JMW75_RS25625 21162..21941 - 780 WP_023287113.1 site-specific integrase -
JMW75_RS25630 22139..23155 - 1017 WP_009309921.1 hypothetical protein -
JMW75_RS25635 23189..23524 - 336 WP_009309920.1 hypothetical protein -
JMW75_RS25640 23574..23714 - 141 WP_162898808.1 hypothetical protein -
JMW75_RS25645 23943..24248 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB -
JMW75_RS25650 24250..24468 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA -
JMW75_RS25655 25065..25295 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JMW75_RS25660 25292..25708 + 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin Toxin
JMW75_RS25665 25749..26627 - 879 WP_032427228.1 restriction endonuclease -
JMW75_RS25670 27289..28566 + 1278 WP_009309915.1 HlyD family secretion protein -
JMW75_RS25675 28556..30664 + 2109 WP_032427226.1 peptidase domain-containing ABC transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 19522..20502 980
- inside Conjugative plasmid - mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..233670 233670


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15154.59 Da        Isoelectric Point: 7.8637

>T290777 WP_020804312.1 NZ_LR890361:25292-25708 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT290777 WP_001261276.1 NZ_LR890361:25065-25295 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3Q8D6L7

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References