Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5142828..5143453 Replicon chromosome
Accession NZ_LR890307
Organism Klebsiella pneumoniae isolate INF188-sc-2280054

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JMW12_RS25135 Protein ID WP_002882817.1
Coordinates 5142828..5143211 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JMW12_RS25140 Protein ID WP_004150355.1
Coordinates 5143211..5143453 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW12_RS25120 5140194..5141096 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JMW12_RS25125 5141093..5141728 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JMW12_RS25130 5141725..5142654 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JMW12_RS25135 5142828..5143211 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JMW12_RS25140 5143211..5143453 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JMW12_RS25145 5143658..5144575 + 918 WP_023302328.1 alpha/beta hydrolase -
JMW12_RS25150 5144589..5145530 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JMW12_RS25155 5145575..5146012 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JMW12_RS25160 5146009..5146869 - 861 WP_004146232.1 virulence factor BrkB family protein -
JMW12_RS25165 5146863..5147462 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T290600 WP_002882817.1 NZ_LR890307:c5143211-5142828 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT290600 WP_004150355.1 NZ_LR890307:c5143453-5143211 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References