Detailed information of TA system TA290575

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 122319..122745
• Replicon: plasmid 2
• Accession: NZ_LR890300
• Organism: Escherichia coli isolate MSB1_3B-sc-2280406

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JM188_RS25265
• Protein ID: WP_001312861.1
• Coordinates: 122319..122477 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	122521..122745 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JM188_RS25230	117431..117658	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
JM188_RS25235	117746..118423	-	678	WP_001348626.1	PAS domain-containing protein	-
JM188_RS25240	118557..118940	-	384	WP_001151566.1	relaxosome protein TraM	-
JM188_RS25245	119271..119873	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
JM188_RS25250	120170..120991	-	822	WP_001234469.1	DUF945 domain-containing protein	-
JM188_RS25255	121110..121397	-	288	WP_000107535.1	hypothetical protein	-
JM188_RS25260	121422..121628	-	207	WP_000275859.1	hypothetical protein	-
JM188_RS25265	122319..122477	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	122521..122745	-	225	NuclAT_0	-	Antitoxin
-	122521..122745	-	225	NuclAT_0	-	Antitoxin
-	122521..122745	-	225	NuclAT_0	-	Antitoxin
-	122521..122745	-	225	NuclAT_0	-	Antitoxin
JM188_RS25270	122557..122745	+	189	WP_001299721.1	hypothetical protein	-
JM188_RS25275	122757..123476	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
JM188_RS25280	123473..123907	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
JM188_RS25285	123962..125920	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
JM188_RS25290	125986..126219	-	234	WP_000005990.1	DUF905 family protein	-
JM188_RS25295	126282..126821	-	540	WP_000290841.1	single-stranded DNA-binding protein	-
JM188_RS25300	127464..127694	-	231	WP_021526513.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaTEM-1B / blaCTX-M-27 / erm(B)	senB	1..160467	160467

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T290575 WP_001312861.1 NZ_LR890300:c122477-122319 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT290575 NZ_LR890300:c122745-122521 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin