Detailed information of TA system TA290440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3960419..3962072
• Replicon: chromosome
• Accession: NZ_LR890270
• Organism: Escherichia coli isolate MINF_7C-sc-2280452

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A0X5EJM1
• Locus tag: JMW01_RS18640
• Protein ID: WP_001615045.1
• Coordinates: 3961188..3962072 (+)
• Length: 295 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: JMW01_RS18635
• Protein ID: WP_001134467.1
• Coordinates: 3960419..3961201 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMW01_RS18610	3955872..3957197	-	1326	WP_001586702.1	DNA phosphorothioation-dependent restriction protein DptG	-
JMW01_RS18615	3957202..3958851	-	1650	WP_001586703.1	DNA phosphorothioation-dependent restriction protein DptF	-
JMW01_RS18620	3959128..3959352	-	225	WP_024187424.1	helix-turn-helix domain-containing protein	-
JMW01_RS18625	3959433..3959663	-	231	WP_001577779.1	helix-turn-helix domain-containing protein	-
JMW01_RS18630	3959732..3960042	+	311	Protein_3629	helix-turn-helix domain-containing protein	-
JMW01_RS18635	3960419..3961201	+	783	WP_001134467.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
JMW01_RS18640	3961188..3962072	+	885	WP_001615045.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
JMW01_RS18645	3962524..3963408	-	885	WP_001119037.1	LysR family transcriptional regulator	-
JMW01_RS18650	3963673..3964731	+	1059	WP_001586705.1	alpha/beta hydrolase	-
JMW01_RS18655	3964873..3966000	+	1128	WP_000171079.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	3948733..3997476	48743

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-209)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 295 a.a.
• Molecular weight: 33151.42 Da
• Isoelectric Point: 7.7408

>T290440 WP_001615045.1 NZ_LR890270:3961188-3962072 [Escherichia coli]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAAINDALKRITASLNGRPGITAIRQENKA
DEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQHPRDLFDVLNMLE
KPGVMREIFDGFLCYLAGHPRPIAELLAPNWDTERITTLYAQEFSGMTQQETSLESLLSVTTLLPQALKSHLTDLDREFS
LSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 885 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29093.39 Da
• Isoelectric Point: 8.4395

>AT290440 WP_001134467.1 NZ_LR890270:3960419-3961201 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVTSLTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGMNLLSPRKLQALLCASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: A0A0X5EJM1