Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 9603..10128 Replicon plasmid 5
Accession NZ_LR883054
Organism Escherichia coli isolate L1_E925

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag JL017_RS25300 Protein ID WP_001159871.1
Coordinates 9823..10128 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag JL017_RS25295 Protein ID WP_000813634.1
Coordinates 9603..9821 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JL017_RS25270 5239..6189 - 951 WP_001699589.1 virulence factor VirK -
JL017_RS25275 6194..7282 - 1089 WP_004100285.1 putative hexosyltransferase CapU -
JL017_RS25280 7285..8127 - 843 WP_160371899.1 polysaccharide deacetylase family protein -
JL017_RS25285 8371..8901 - 531 WP_011387704.1 hypothetical protein -
JL017_RS25290 8901..9158 - 258 WP_000343092.1 hypothetical protein -
JL017_RS25295 9603..9821 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JL017_RS25300 9823..10128 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
JL017_RS25305 10129..10842 + 714 WP_000016957.1 site-specific integrase -
JL017_RS25310 11172..11813 + 642 WP_000470631.1 recombinase family protein -
JL017_RS25315 12253..12933 - 681 WP_000452091.1 EAL domain-containing protein -
JL017_RS25320 13181..13884 + 704 Protein_19 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espC / espC 1..51418 51418
- flank IS/Tn - - 11190..11813 623


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T290164 WP_001159871.1 NZ_LR883054:9823-10128 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT290164 WP_000813634.1 NZ_LR883054:9603-9821 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References