Detailed information of TA system TA028885

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1241164..1241732
• Replicon: chromosome
• Accession: NC_020245
• Organism: Mycobacterium bovis BCG str. Korea 1168P

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: K60_RS05910
• Protein ID: WP_003405865.1
• Coordinates: 1241358..1241732 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: K60_RS05905
• Protein ID: WP_003405863.1
• Coordinates: 1241164..1241361 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K60_RS05885	1237205..1237843	-	639	WP_011799158.1	lipid droplet-associated protein	-
K60_RS05890	1237933..1238940	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
K60_RS05895	1238957..1239940	-	984	WP_003898731.1	hypothetical protein	-
K60_RS05900	1240003..1241076	+	1074	WP_003405860.1	redox-regulated ATPase YchF	-
K60_RS05905	1241164..1241361	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K60_RS05910	1241358..1241732	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
K60_RS05915	1241935..1242633	+	699	WP_010950494.1	hypothetical protein	-
K60_RS05920	1242751..1242936	+	186	WP_003901093.1	hypothetical protein	-
K60_RS05925	1242863..1243138	-	276	WP_003405867.1	hypothetical protein	-
K60_RS05930	1243381..1243704	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
K60_RS05935	1243719..1244429	-	711	WP_003911399.1	hypothetical protein	-
K60_RS22470	1244451..1244660	+	210	WP_003911400.1	hypothetical protein	-
K60_RS05945	1244612..1245319	-	708	Protein_1158	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T28885 WP_003405865.1 NC_020245:1241358-1241732 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T28885 NC_020245:1241358-1241732 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT28885 WP_003405863.1 NC_020245:1241164-1241361 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT28885 NC_020245:1241164-1241361 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07