Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 744806..745533 Replicon chromosome
Accession NZ_LR213455
Organism Shigella flexneri strain AUSMDU00008332 isolate AUSMDU00008332

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag E0E35_RS03770 Protein ID WP_000550189.1
Coordinates 744806..745120 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag E0E35_RS03775 Protein ID WP_000560266.1
Coordinates 745117..745533 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E0E35_RS03750 (740951) 740951..741949 - 999 WP_005050984.1 Gfo/Idh/MocA family oxidoreductase -
E0E35_RS03755 (742028) 742028..742720 - 693 WP_000942538.1 vancomycin high temperature exclusion protein -
E0E35_RS03760 (742797) 742797..743300 - 504 WP_005050988.1 M48 family metallopeptidase -
E0E35_RS03765 (743385) 743385..744521 + 1137 WP_000018656.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
E0E35_RS03770 (744806) 744806..745120 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
E0E35_RS03775 (745117) 745117..745533 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
E0E35_RS03780 (745578) 745578..747596 - 2019 WP_000121487.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
E0E35_RS03785 (747822) 747822..750173 - 2352 WP_000695506.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T288266 WP_000550189.1 NZ_LR213455:744806-745120 [Shigella flexneri]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT288266 WP_000560266.1 NZ_LR213455:745117-745533 [Shigella flexneri]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References