288088

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 12380..13488 Replicon plasmid 5
Accession NZ_LR135239
Organism Enterococcus faecium isolate E7067

Toxin (Protein)


Gene name MNTss Uniprot ID A0A0M4MHJ8
Locus tag EQB63_RS15810 Protein ID WP_002303392.1
Coordinates 12619..13488 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag EQB63_RS15805 Protein ID WP_002303393.1
Coordinates 12380..12604 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB63_RS15785 8201..8881 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
EQB63_RS15790 8936..9871 + 936 Protein_11 ABC-F type ribosomal protection protein Lsa(E) -
EQB63_RS15795 9925..10728 + 804 WP_002294514.1 lincosamide nucleotidyltransferase Lnu(B) -
EQB63_RS15800 11416..12237 + 822 Protein_13 recombinase zinc beta ribbon domain-containing protein -
EQB63_RS15805 12380..12604 + 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
EQB63_RS15810 12619..13488 + 870 WP_002303392.1 nucleotidyltransferase domain-containing protein Toxin
EQB63_RS15815 13469..14203 + 735 WP_000662263.1 class I SAM-dependent methyltransferase -
EQB63_RS15820 14236..15144 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
EQB63_RS15825 15141..15389 + 249 WP_002347175.1 hypothetical protein -
EQB63_RS15830 15392..16300 - 909 WP_002324522.1 IS982-like element ISEfm1 family transposase -
EQB63_RS15835 16401..17696 - 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
EQB63_RS15840 17941..18192 + 252 Protein_21 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX / lnu(B) / ant(6)-Ia / aph(3')-III / erm(B) - 1..51960 51960
- inside IScluster/Tn VanHAX / lnu(B) / ant(6)-Ia / aph(3')-III / erm(B) - 4313..48735 44422


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32807.38 Da        Isoelectric Point: 4.7269

>T288088 WP_002303392.1 NZ_LR135239:12619-13488 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT288088 WP_002303393.1 NZ_LR135239:12380-12604 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MHJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References