Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 917189..917843 Replicon chromosome
Accession NZ_LR134248
Organism Escherichia coli strain NCTC10537

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag EL141_RS04475 Protein ID WP_000244783.1
Coordinates 917436..917843 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EL141_RS04470 Protein ID WP_000354046.1
Coordinates 917189..917455 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL141_RS04445 912357..913100 + 744 WP_000951948.1 SDR family oxidoreductase -
EL141_RS04450 913157..914590 - 1434 WP_012775976.1 6-phospho-beta-glucosidase BglA -
EL141_RS04455 914635..914946 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
EL141_RS04460 915110..915769 + 660 WP_000250274.1 hemolysin III family protein -
EL141_RS04465 915965..916946 - 982 Protein_869 tRNA-modifying protein YgfZ -
EL141_RS04470 917189..917455 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EL141_RS04475 917436..917843 + 408 WP_000244783.1 protein YgfX Toxin
EL141_RS04480 917883..918404 - 522 WP_001055874.1 flavodoxin FldB -
EL141_RS04485 918516..919412 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EL141_RS04490 919437..920147 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EL141_RS04495 920153..921886 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T287380 WP_000244783.1 NZ_LR134248:917436-917843 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T287380 NZ_LR134248:917436-917843 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTAGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT287380 WP_000354046.1 NZ_LR134248:917189-917455 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT287380 NZ_LR134248:917189-917455 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References