287245

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 4425784..4426621 Replicon chromosome
Accession NZ_LR134234
Organism Escherichia coli strain NCTC8623

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag EL131_RS21575 Protein ID WP_000227784.1
Coordinates 4425784..4426326 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag EL131_RS21580 Protein ID WP_001297137.1
Coordinates 4426310..4426621 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL131_RS21555 4421323..4422234 - 912 WP_000705874.1 2-dehydropantoate 2-reductase -
EL131_RS21560 4422402..4422893 + 492 WP_001138904.1 nucleotide binding protein YajQ -
EL131_RS21565 4423021..4424385 - 1365 WP_001000974.1 MFS transporter -
EL131_RS21570 4424793..4425728 + 936 WP_001365761.1 sel1 repeat family protein -
EL131_RS21575 4425784..4426326 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
EL131_RS21580 4426310..4426621 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
EL131_RS21585 4426806..4427696 - 891 WP_000971336.1 heme o synthase -
EL131_RS21590 4427708..4428037 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
EL131_RS21595 4428037..4428651 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
EL131_RS21600 4428641..4430632 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
EL131_RS21605 4430654..4431601 - 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T287245 WP_000227784.1 NZ_LR134234:c4426326-4425784 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT287245 WP_001297137.1 NZ_LR134234:c4426621-4426310 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References