Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2226497..2227151 Replicon chromosome
Accession NZ_LR134234
Organism Escherichia coli strain NCTC8623

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag EL131_RS10935 Protein ID WP_000244781.1
Coordinates 2226497..2226904 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EL131_RS10940 Protein ID WP_000354046.1
Coordinates 2226885..2227151 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL131_RS10915 2222454..2224187 - 1734 WP_000813218.1 single-stranded-DNA-specific exonuclease RecJ -
EL131_RS10920 2224193..2224903 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EL131_RS10925 2224928..2225824 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EL131_RS10930 2225936..2226457 + 522 WP_001055874.1 flavodoxin FldB -
EL131_RS10935 2226497..2226904 - 408 WP_000244781.1 protein YgfX Toxin
EL131_RS10940 2226885..2227151 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EL131_RS10945 2227394..2228374 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
EL131_RS10950 2228570..2229229 - 660 WP_000250274.1 hemolysin III family protein -
EL131_RS10955 2229393..2229704 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
EL131_RS10960 2229749..2231182 + 1434 WP_024186065.1 6-phospho-beta-glucosidase BglA -
EL131_RS10965 2231239..2231982 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T287238 WP_000244781.1 NZ_LR134234:c2226904-2226497 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT287238 WP_000354046.1 NZ_LR134234:c2227151-2226885 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References