Detailed information of TA system TA028696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 507441..508068
• Replicon: chromosome
• Accession: NC_019970
• Organism: Thermoanaerobacterium thermosaccharolyticum M0795

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: I3VUG0
• Locus tag: THETHE_RS02555
• Protein ID: WP_014758038.1
• Coordinates: 507718..508068 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I3VUF9
• Locus tag: THETHE_RS02550
• Protein ID: WP_013297076.1
• Coordinates: 507441..507716 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THETHE_RS02530	503208..503570	+	363	WP_013297072.1	hypothetical protein	-
THETHE_RS02535	503650..505176	+	1527	WP_013297073.1	bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase	-
THETHE_RS02540	505205..506173	+	969	WP_155983727.1	outer membrane lipoprotein carrier protein LolA	-
THETHE_RS02545	506187..507353	+	1167	WP_015310976.1	alanine racemase	-
THETHE_RS02550	507441..507716	+	276	WP_013297076.1	ribbon-helix-helix protein, CopG family	Antitoxin
THETHE_RS02555	507718..508068	+	351	WP_014758038.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
THETHE_RS02560	508317..509555	-	1239	WP_196795815.1	IS110 family transposase	-
THETHE_RS02565	510062..510511	+	450	WP_015310978.1	hypothetical protein	-
THETHE_RS02570	510632..512653	+	2022	WP_015310979.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12931.00 Da
• Isoelectric Point: 6.4714

>T28696 WP_014758038.1 NC_019970:507718-508068 [Thermoanaerobacterium thermosaccharolyticum M0795]
MIVKRGDILYADLSPVIGSEQGGVRPVLVIQNDIGNKYSPTVIVSAITSQINKAKLPTHVEINGTEYGLNKDSVILLEQI
RTIDKKRLREKIGHFDQEMMEKVNEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T28696 NC_019970:507718-508068 [Thermoanaerobacterium thermosaccharolyticum M0795]
ATGATTGTAAAACGTGGAGATATTTTGTATGCTGATTTAAGCCCTGTCATAGGCTCAGAACAGGGTGGTGTTAGGCCCGT
ACTTGTCATACAGAACGATATCGGCAATAAGTACAGCCCTACAGTTATTGTGTCAGCAATAACATCACAGATAAACAAGG
CTAAATTGCCGACACATGTTGAGATAAATGGAACGGAATACGGTCTTAATAAAGATTCGGTTATTTTATTAGAGCAGATA
AGGACTATAGACAAAAAGCGGCTAAGGGAAAAGATAGGTCATTTTGACCAAGAGATGATGGAAAAGGTAAATGAGGCATT
GCAAATAAGCCTTGGGTTGATAGATTTTTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10514.31 Da
• Isoelectric Point: 7.7813

>AT28696 WP_013297076.1 NC_019970:507441-507716 [Thermoanaerobacterium thermosaccharolyticum M0795]
VGETKRILVSLPESLLEEVDVLAAMENRNRSEFIREAMKLYIKERKKMKIRESMKKGYLEMAAINAELAELGLTADNECF
NGYEKKLKKCD

Nucleotide

• Length: 276 bp

>AT28696 NC_019970:507441-507716 [Thermoanaerobacterium thermosaccharolyticum M0795]
GTGGGCGAGACAAAAAGAATACTCGTCAGTTTGCCGGAAAGCTTACTGGAAGAAGTTGATGTACTTGCTGCTATGGAAAA
TAGAAACCGCAGTGAATTTATAAGAGAAGCCATGAAATTGTACATTAAAGAGAGAAAGAAGATGAAGATAAGGGAGAGCA
TGAAAAAAGGGTATCTTGAGATGGCGGCTATAAATGCAGAACTTGCGGAATTGGGCCTAACCGCTGACAACGAATGTTTT
AACGGATACGAGAAAAAATTAAAAAAGTGTGATTGA

Structures

Toxin

• AlphaFold DB: I3VUG0

Antitoxin

• AlphaFold DB: I3VUF9