286663

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3700919..3701756 Replicon chromosome
Accession NZ_LR134080
Organism Escherichia coli strain NCTC9082

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag EL023_RS18270 Protein ID WP_000227784.1
Coordinates 3701214..3701756 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag EL023_RS18265 Protein ID WP_001297137.1
Coordinates 3700919..3701230 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL023_RS18240 3695939..3696886 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
EL023_RS18245 3696908..3698899 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
EL023_RS18250 3698889..3699503 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
EL023_RS18255 3699503..3699832 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
EL023_RS18260 3699844..3700734 + 891 WP_000971336.1 heme o synthase -
EL023_RS18265 3700919..3701230 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
EL023_RS18270 3701214..3701756 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
EL023_RS18275 3701812..3702747 - 936 WP_001297127.1 sel1 repeat family protein -
EL023_RS18280 3703155..3704519 + 1365 WP_001000974.1 MFS transporter -
EL023_RS18285 3704647..3705138 - 492 WP_001138904.1 nucleotide binding protein YajQ -
EL023_RS18290 3705306..3706217 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T286663 WP_000227784.1 NZ_LR134080:3701214-3701756 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT286663 WP_001297137.1 NZ_LR134080:3700919-3701230 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References