Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 4756824..4757515 Replicon chromosome
Accession NZ_LR130541
Organism Klebsiella pneumoniae strain AJ218 isolate AJ218

Toxin (Protein)


Gene name ykfI Uniprot ID A0A483YFJ1
Locus tag EW045_RS23595 Protein ID WP_025987721.1
Coordinates 4756824..4757165 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A2A5MHA6
Locus tag EW045_RS23600 Protein ID WP_019725272.1
Coordinates 4757189..4757515 (-) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW045_RS23585 4753525..4755249 - 1725 WP_048269794.1 retron Ec67 family RNA-directed DNA polymerase/endonuclease -
EW045_RS23590 4755876..4756709 - 834 WP_048269810.1 DUF4942 domain-containing protein -
EW045_RS23595 4756824..4757165 - 342 WP_025987721.1 TA system toxin CbtA family protein Toxin
EW045_RS23600 4757189..4757515 - 327 WP_019725272.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
EW045_RS23605 4757529..4758005 - 477 WP_048269792.1 DNA repair protein RadC -
EW045_RS23610 4758016..4758462 - 447 WP_048269791.1 antirestriction protein -
EW045_RS23615 4758686..4759375 - 690 WP_048269790.1 hypothetical protein -
EW045_RS23620 4759695..4760048 + 354 WP_048269789.1 hypothetical protein -
EW045_RS23625 4760085..4760975 - 891 WP_048269788.1 50S ribosome-binding GTPase -
EW045_RS23630 4761058..4762146 - 1089 WP_048269787.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4739779..4775715 35936


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-107)

Antitoxin

Antitoxin CbeA

(8-107)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12867.90 Da        Isoelectric Point: 8.5195

>T286313 WP_025987721.1 NZ_LR130541:c4757165-4756824 [Klebsiella pneumoniae]
MKTLPATIQRAAKPCPSPVTVWQMLLTRLLEQHYGLTLNDTPFSDETVIQEHINAGITLVDAVNFLVEKYELVRIDRRGF
NCQEQSPYLGAVDILRARQATGLLRQRHLPSIR

Download         Length: 342 bp

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12276.01 Da        Isoelectric Point: 6.9560

>AT286313 WP_019725272.1 NZ_LR130541:c4757515-4757189 [Klebsiella pneumoniae]
MPLTDAIQWGLKRSFTPNFGARLVQEGNRLHYLADRANITGKFGDAECLKLEEAFPHFIRQMELMLSTGELNPRHAHSVT
LYHNAFTCEADTLGSCGYVYIVIYPTQR

Download         Length: 327 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483YFJ1

Antitoxin

Source ID Structure
AlphaFold DB A0A2A5MHA6

References