Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 84906..85507 Replicon plasmid pT309.Ta3_1
Accession NZ_CP128256
Organism Escherichia coli strain T309.Ta3

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag QRX22_RS25820 Protein ID WP_001216034.1
Coordinates 84906..85286 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag QRX22_RS25825 Protein ID WP_001190712.1
Coordinates 85286..85507 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRX22_RS25790 (QRX22_25790) 80032..80133 - 102 Protein_80 transcriptional regulator -
QRX22_RS25795 (QRX22_25795) 80347..81831 - 1485 WP_000124159.1 hypothetical protein -
QRX22_RS25800 (QRX22_25800) 81831..83024 - 1194 WP_000219618.1 hypothetical protein -
QRX22_RS25805 (QRX22_25805) 83110..83562 - 453 WP_001326849.1 late promoter-activating protein -
QRX22_RS25810 (QRX22_25810) 83651..84694 - 1044 WP_000648833.1 DUF968 domain-containing protein -
QRX22_RS25815 (QRX22_25815) 84722..84901 - 180 WP_000113018.1 hypothetical protein -
QRX22_RS25820 (QRX22_25820) 84906..85286 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
QRX22_RS25825 (QRX22_25825) 85286..85507 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
QRX22_RS25830 (QRX22_25830) 85580..85774 - 195 WP_050516570.1 hypothetical protein -
QRX22_RS25835 (QRX22_25835) 86035..89841 - 3807 WP_077875973.1 autotransporter outer membrane beta-barrel domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / sul1 / qacE / aadA5 / catB3 / dfrA1 - 1..113750 113750


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T284480 WP_001216034.1 NZ_CP128256:c85286-84906 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T284480 NZ_CP128256:c85286-84906 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT284480 WP_001190712.1 NZ_CP128256:c85507-85286 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT284480 NZ_CP128256:c85507-85286 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGAAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References