Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3035404..3035966 Replicon chromosome
Accession NZ_CP127314
Organism Escherichia coli strain C25

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag QRM69_RS14620 Protein ID WP_000605675.1
Coordinates 3035404..3035682 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag QRM69_RS14625 Protein ID WP_000781370.1
Coordinates 3035682..3035966 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRM69_RS14595 (3030990) 3030990..3031421 - 432 WP_000152307.1 peroxiredoxin OsmC -
QRM69_RS14600 (3031766) 3031766..3031981 + 216 WP_000495766.1 biofilm-dependent modulation protein -
QRM69_RS14605 (3032083) 3032083..3032220 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
QRM69_RS14610 (3032377) 3032377..3034074 + 1698 WP_000433460.1 malate dehydrogenase -
QRM69_RS14615 (3034208) 3034208..3035218 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
QRM69_RS14620 (3035404) 3035404..3035682 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QRM69_RS14625 (3035682) 3035682..3035966 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
QRM69_RS14630 (3036195) 3036195..3036848 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
QRM69_RS14635 (3036841) 3036841..3037725 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
QRM69_RS14640 (3037738) 3037738..3040785 - 3048 WP_286005070.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T284151 WP_000605675.1 NZ_CP127314:3035404-3035682 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT284151 WP_000781370.1 NZ_CP127314:3035682-3035966 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References