Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 17120..17918 Replicon plasmid pEND_Eco 14033-3
Accession NZ_CP126951
Organism Escherichia coli O78:H4 strain APEC E14033

Toxin (Protein)


Gene name ataT Uniprot ID -
Locus tag QQA23_RS25915 Protein ID WP_232602840.1
Coordinates 17397..17918 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A141BRA2
Locus tag QQA23_RS25910 Protein ID WP_001711191.1
Coordinates 17120..17389 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA23_RS25885 (QQA23_25885) 12546..13886 - 1341 WP_077136336.1 DnaB-like helicase C-terminal domain-containing protein -
QQA23_RS25890 (QQA23_25890) 13930..14670 - 741 WP_021520120.1 hypothetical protein -
QQA23_RS25895 (QQA23_25895) 14953..15720 + 768 WP_000342417.1 hypothetical protein -
QQA23_RS25900 (QQA23_25900) 15773..16126 - 354 WP_160394000.1 hypothetical protein -
QQA23_RS25905 (QQA23_25905) 16132..16800 - 669 WP_000161228.1 division plane positioning ATPase MipZ -
QQA23_RS25910 (QQA23_25910) 17120..17389 + 270 WP_001711191.1 DUF1778 domain-containing protein Antitoxin
QQA23_RS25915 (QQA23_25915) 17397..17918 + 522 WP_232602840.1 GNAT family N-acetyltransferase Toxin
QQA23_RS25920 (QQA23_25920) 18086..18337 - 252 WP_001404395.1 hypothetical protein -
QQA23_RS25925 (QQA23_25925) 18339..19031 - 693 WP_012640731.1 hypothetical protein -
QQA23_RS25930 (QQA23_25930) 19045..19368 - 324 WP_001717323.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..108542 108542


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19434.25 Da        Isoelectric Point: 8.6369

>T283332 WP_232602840.1 NZ_CP126951:17397-17918 [Escherichia coli O78:H4]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLVNHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLIGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10054.53 Da        Isoelectric Point: 8.7353

>AT283332 WP_001711191.1 NZ_CP126951:17120-17389 [Escherichia coli O78:H4]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A141BRA2

References