Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 66840..67399 Replicon plasmid pAA-09EL50
Accession NC_018654
Organism Escherichia coli O104:H4 str. 2009EL-2050

Toxin (Protein)


Gene name relE Uniprot ID G4VUF8
Locus tag O3M_RS27570 Protein ID WP_000421273.1
Coordinates 67124..67399 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag O3M_RS27565 Protein ID WP_001387467.1
Coordinates 66840..67124 (+) Length 95 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3M_RS27530 61879..62556 - 678 WP_001339397.1 IS66 family insertion sequence hypothetical protein -
O3M_RS27535 62621..62833 + 213 Protein_72 ANR family transcriptional regulator -
O3M_RS27540 63284..64264 - 981 WP_000019427.1 IS5-like element ISKpn26 family transposase -
O3M_RS27545 64545..64799 + 255 WP_000083850.1 replication regulatory protein RepA -
O3M_RS32190 64906..65040 + 135 Protein_75 protein CopA/IncA -
O3M_RS27550 65037..65111 + 75 WP_014966221.1 RepA leader peptide Tap -
O3M_RS27560 65104..65932 + 829 Protein_77 incFII family plasmid replication initiator RepA -
O3M_RS27565 66840..67124 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
O3M_RS27570 67124..67399 + 276 WP_000421273.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
O3M_RS27575 67405..67736 + 332 Protein_80 type II toxin-antitoxin system ParD family antitoxin -
O3M_RS27580 67852..68013 + 162 WP_001440647.1 type II toxin-antitoxin system RelE/ParE family toxin -
O3M_RS27585 68136..68261 + 126 WP_032159539.1 hypothetical protein -
O3M_RS27590 68382..69122 + 741 WP_001066951.1 tyrosine-type recombinase/integrase -
O3M_RS27595 69401..70378 - 978 WP_001387465.1 RepB family plasmid replication initiator protein -
O3M_RS27610 71470..71619 + 150 Protein_85 transposase domain-containing protein -
O3M_RS27615 71702..72103 - 402 WP_001392130.1 type II toxin-antitoxin system VapC family toxin -
O3M_RS27620 72127..72357 - 231 WP_001261288.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - aggD / aggC / aggB / aggA / aap/aspU 1..74213 74213


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 92 a.a.        Molecular weight: 10765.45 Da        Isoelectric Point: 9.9324

>T28305 WP_000421273.1 NC_018654:67124-67399 [Escherichia coli O104:H4 str. 2009EL-2050]
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T28305 NC_018654:67124-67399 [Escherichia coli O104:H4 str. 2009EL-2050]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT28305 WP_001387467.1 NC_018654:66840-67124 [Escherichia coli O104:H4 str. 2009EL-2050]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT28305 NC_018654:66840-67124 [Escherichia coli O104:H4 str. 2009EL-2050]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0E0Y6H1


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References