Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2215438..2216024 Replicon chromosome
Accession NZ_CP125620
Organism Mycobacterium tuberculosis strain BLR 4299 2019

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag O3Q54_RS10395 Protein ID WP_003410010.1
Coordinates 2215438..2215782 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag O3Q54_RS10400 Protein ID WP_003410014.1
Coordinates 2215776..2216024 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3Q54_RS10360 (O3Q54_10360) 2211144..2211743 + 600 WP_003409989.1 L-lysine exporter -
O3Q54_RS10365 (O3Q54_10365) 2212159..2212587 + 429 WP_003906713.1 cellulose-binding protein -
O3Q54_RS10370 (O3Q54_10370) 2212813..2213352 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
O3Q54_RS10375 (O3Q54_10375) 2213872..2214432 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
O3Q54_RS10380 (O3Q54_10380) 2214429..2214770 - 342 WP_003410003.1 DUF2384 domain-containing protein -
O3Q54_RS10385 (O3Q54_10385) 2214856..2215113 + 258 WP_003410006.1 hypothetical protein -
O3Q54_RS10390 (O3Q54_10390) 2215014..2215349 - 336 WP_003410009.1 dehydrogenase -
O3Q54_RS10395 (O3Q54_10395) 2215438..2215782 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
O3Q54_RS10400 (O3Q54_10400) 2215776..2216024 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
O3Q54_RS10405 (O3Q54_10405) 2216124..2218439 - 2316 WP_003899120.1 cation transporter ATPase CptG -
O3Q54_RS10410 (O3Q54_10410) 2218436..2218708 - 273 WP_003410017.1 DUF1490 family protein -
O3Q54_RS10415 (O3Q54_10415) 2218761..2219117 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
O3Q54_RS10420 (O3Q54_10420) 2219274..2220041 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T282197 WP_003410010.1 NZ_CP125620:c2215782-2215438 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT282197 WP_003410014.1 NZ_CP125620:c2216024-2215776 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References