Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 485011..485648 Replicon chromosome
Accession NZ_CP125279
Organism Bacillus velezensis strain FLU-1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QLH34_RS02490 Protein ID WP_003156187.1
Coordinates 485298..485648 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag QLH34_RS02485 Protein ID WP_003156188.1
Coordinates 485011..485292 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QLH34_RS02465 (481376) 481376..481975 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
QLH34_RS02470 (482068) 482068..482433 + 366 WP_007410229.1 holo-ACP synthase -
QLH34_RS02475 (482598) 482598..483605 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
QLH34_RS02480 (483722) 483722..484891 + 1170 WP_025284272.1 alanine racemase -
QLH34_RS02485 (485011) 485011..485292 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QLH34_RS02490 (485298) 485298..485648 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QLH34_RS02495 (485766) 485766..486587 + 822 WP_003156182.1 STAS domain-containing protein -
QLH34_RS02500 (486592) 486592..486957 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
QLH34_RS02505 (486960) 486960..487361 + 402 WP_003156178.1 anti-sigma regulatory factor -
QLH34_RS02510 (487373) 487373..488380 + 1008 WP_052827076.1 PP2C family protein-serine/threonine phosphatase -
QLH34_RS02515 (488444) 488444..488773 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
QLH34_RS02520 (488770) 488770..489252 + 483 WP_003156173.1 anti-sigma B factor RsbW -
QLH34_RS02525 (489218) 489218..490006 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
QLH34_RS02530 (490006) 490006..490608 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T281951 WP_003156187.1 NZ_CP125279:485298-485648 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT281951 WP_003156188.1 NZ_CP125279:485011-485292 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References