Detailed information of TA system TA028144

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 5721708..5722349
• Replicon: chromosome
• Accession: NC_018289
• Organism: Mycobacterium smegmatis str. MC2 155

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MSMEI_RS27575
• Protein ID: WP_011730672.1
• Coordinates: 5721708..5722151 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: I7FKW8
• Locus tag: MSMEI_RS27580
• Protein ID: WP_011730673.1
• Coordinates: 5722161..5722349 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MSMEI_RS27555	5717191..5717928	+	738	WP_011730668.1	GntR family transcriptional regulator	-
MSMEI_RS27560	5717930..5719462	+	1533	WP_011730669.1	cyclohexanecarboxylate-CoA ligase	-
MSMEI_RS27565	5719463..5720236	+	774	WP_011730670.1	SDR family oxidoreductase	-
MSMEI_RS27570	5720233..5721705	+	1473	WP_011730671.1	long-chain fatty acid--CoA ligase	-
MSMEI_RS27575	5721708..5722151	-	444	WP_011730672.1	SRPBCC family protein	Toxin
MSMEI_RS27580	5722161..5722349	-	189	WP_011730673.1	antitoxin	Antitoxin
MSMEI_RS27585	5722375..5724750	-	2376	WP_011730674.1	cation-translocating P-type ATPase	-
MSMEI_RS27590	5724764..5726371	-	1608	WP_162139558.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-141)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16157.75 Da
• Isoelectric Point: 9.9432

>T28144 WP_011730672.1 NC_018289:c5722151-5721708 [Mycolicibacterium smegmatis MC2 155]
MAKLSVSVEVPLPPEQAWEYASDLSRYHEWLSIHRAWRSKLPETLEKGTVIESIVEVKGMLNRVRWTLVHYKPPQALTLN
GDGRGGVKVKLIGKIKPTDNGATVGFDLHLGGPALFGPIGMVVAAALKSDIQESLNRFKQLYAPSAT

Nucleotide

• Length: 444 bp

>T28144 NC_018289:c5722151-5721708 [Mycolicibacterium smegmatis MC2 155]
ATGGCCAAACTTTCCGTCTCCGTCGAAGTTCCATTGCCGCCGGAGCAGGCGTGGGAGTATGCCTCCGACCTGTCCCGGTA
CCACGAGTGGCTCAGCATTCACCGGGCCTGGCGCTCGAAGCTGCCCGAGACCCTGGAGAAGGGCACGGTGATCGAGTCGA
TCGTCGAGGTCAAGGGCATGCTCAACCGGGTCAGGTGGACGTTGGTCCACTACAAGCCCCCGCAGGCGCTCACACTCAAC
GGTGACGGCCGCGGCGGGGTCAAGGTCAAGCTGATCGGCAAGATCAAGCCCACCGACAACGGCGCGACGGTGGGCTTCGA
CCTGCACCTCGGCGGCCCAGCCCTGTTCGGGCCCATCGGGATGGTGGTGGCCGCGGCGCTCAAGAGCGACATTCAGGAGT
CGCTCAACCGCTTCAAGCAGCTCTACGCCCCGTCCGCGACCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6867.59 Da
• Isoelectric Point: 4.6868

>AT28144 WP_011730673.1 NC_018289:c5722349-5722161 [Mycolicibacterium smegmatis MC2 155]
MGFLDKAKDLLSQNADKVDTVIDKAGDMIDEKTQGKYAQHVDKAQDAAKNAIHKDDPQEPQQ

Nucleotide

• Length: 189 bp

>AT28144 NC_018289:c5722349-5722161 [Mycolicibacterium smegmatis MC2 155]
ATGGGATTCCTGGACAAGGCCAAAGACCTCTTGTCGCAGAACGCCGACAAGGTGGACACCGTCATTGACAAGGCCGGCGA
CATGATCGACGAGAAGACGCAGGGCAAGTACGCCCAGCACGTCGACAAGGCGCAGGACGCCGCGAAGAACGCCATCCACA
AAGACGACCCTCAAGAACCACAGCAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A653F9V2