Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 4530496..4531037 Replicon chromosome
Accession NC_018289
Organism Mycobacterium smegmatis str. MC2 155

Toxin (Protein)


Gene name mazF Uniprot ID A0A2U9PVI3
Locus tag MSMEI_RS21765 Protein ID WP_003895797.1
Coordinates 4530708..4531037 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0R0N3
Locus tag MSMEI_RS21760 Protein ID WP_011729843.1
Coordinates 4530496..4530705 (+) Length 70 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MSMEI_RS21735 4525767..4526537 - 771 WP_011729837.1 SDR family oxidoreductase -
MSMEI_RS21740 4526567..4527475 - 909 WP_011729838.1 cupin domain-containing protein -
MSMEI_RS21745 4527505..4528425 - 921 WP_011729840.1 NADP-dependent oxidoreductase -
MSMEI_RS21750 4528515..4528706 - 192 Protein_4259 hypothetical protein -
MSMEI_RS21755 4528923..4530335 + 1413 Protein_4260 dihydrolipoyl dehydrogenase -
MSMEI_RS21760 4530496..4530705 + 210 WP_011729843.1 antitoxin MazE family protein Antitoxin
MSMEI_RS21765 4530708..4531037 + 330 WP_003895797.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MSMEI_RS21770 4531080..4531643 - 564 WP_014878038.1 TetR/AcrR family transcriptional regulator -
MSMEI_RS21775 4531749..4532684 + 936 WP_003895799.1 alpha/beta fold hydrolase -
MSMEI_RS21780 4532681..4534393 + 1713 WP_011729844.1 NAD(P)/FAD-dependent oxidoreductase -
MSMEI_RS21790 4534610..4534990 + 381 WP_029104585.1 phage holin family protein -
MSMEI_RS21795 4535008..4536033 + 1026 WP_014878040.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-104)

Antitoxin

Antitoxin MazE-like

(5-68)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 11739.52 Da        Isoelectric Point: 10.4023

>T28143 WP_003895797.1 NC_018289:4530708-4531037 [Mycolicibacterium smegmatis MC2 155]
VRRGDIYTAAARGAYTGKPRPVLIIQDDRFDATASVTVVPFTTSDTDAPLLRIRIEPTPATGLSTVSSLMIDKVTTVPRS
SLTHRVGRLAETDMVRTDRALLVFLGIAG

Download         Length: 330 bp

>T28143 NC_018289:4530708-4531037 [Mycolicibacterium smegmatis MC2 155]
GTGCGGCGCGGCGATATCTACACCGCGGCGGCGCGAGGTGCGTACACCGGCAAGCCTCGGCCGGTCCTCATCATCCAGGA
CGACCGATTCGATGCCACCGCGTCGGTCACCGTCGTGCCGTTTACGACAAGCGACACCGACGCACCGCTTCTGCGAATTC
GAATCGAGCCAACGCCAGCCACCGGACTCTCCACGGTCAGCTCGTTGATGATCGACAAGGTGACCACAGTTCCCCGCTCC
AGCTTGACGCACCGTGTCGGCCGACTGGCGGAGACAGACATGGTCAGGACCGACCGCGCACTCCTGGTTTTTCTGGGAAT
CGCCGGGTGA

Antitoxin


Download         Length: 70 a.a.        Molecular weight: 8114.98 Da        Isoelectric Point: 6.2231

>AT28143 WP_011729843.1 NC_018289:4530496-4530705 [Mycolicibacterium smegmatis MC2 155]
MTPARDRVRRHRERLRRQGLRPVQIWVPDVNAPEFRREAHRQSELVAAGEHEAEDQAFVDAISVDWDDA

Download         Length: 210 bp

>AT28143 NC_018289:4530496-4530705 [Mycolicibacterium smegmatis MC2 155]
ATGACTCCCGCGCGTGACCGAGTACGCCGACATCGCGAGCGGCTGCGGCGACAAGGTCTGCGGCCGGTGCAGATCTGGGT
CCCCGACGTGAATGCACCCGAATTTCGCCGGGAGGCGCACCGGCAGTCTGAGTTGGTCGCCGCCGGCGAGCATGAGGCCG
AAGATCAAGCTTTCGTCGACGCGATCTCCGTCGACTGGGACGACGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U9PVI3

Antitoxin

Source ID Structure
AlphaFold DB A0R0N3

References