Detailed information of TA system TA028118

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3240107..3240703
• Replicon: chromosome
• Accession: NC_018177
• Organism: Pseudomonas stutzeri DSM 10701

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PSJM300_RS14910
• Protein ID: WP_014853817.1
• Coordinates: 3240425..3240703 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1C2E4A9
• Locus tag: PSJM300_RS14905
• Protein ID: WP_014853816.1
• Coordinates: 3240107..3240412 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSJM300_RS14875	3235145..3236272	-	1128	WP_014853811.1	metallophosphoesterase	-
PSJM300_RS14880	3236523..3236714	-	192	WP_014853812.1	hypothetical protein	-
PSJM300_RS14885	3236777..3237685	-	909	WP_014853813.1	LysR family transcriptional regulator	-
PSJM300_RS14890	3237787..3239007	+	1221	WP_014853814.1	MFS transporter	-
PSJM300_RS14895	3239030..3239830	+	801	WP_014853815.1	2,5-didehydrogluconate reductase DkgB	-
PSJM300_RS14905	3240107..3240412	-	306	WP_014853816.1	HigA family addiction module antidote protein	Antitoxin
PSJM300_RS14910	3240425..3240703	-	279	WP_014853817.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PSJM300_RS14915	3240758..3241753	-	996	WP_014853818.1	tyrosine-type recombinase/integrase	-
PSJM300_RS14920	3241750..3243030	-	1281	WP_014853819.1	hypothetical protein	-
PSJM300_RS14925	3243162..3244349	-	1188	WP_014853820.1	zonular occludens toxin	-
PSJM300_RS14930	3244359..3244685	-	327	WP_014853821.1	DUF2523 domain-containing protein	-
PSJM300_RS14935	3244685..3244984	-	300	WP_014853822.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3240107..3251449	11342

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10516.89 Da
• Isoelectric Point: 7.8943

>T28118 WP_014853817.1 NC_018177:c3240703-3240425 [Pseudomonas stutzeri DSM 10701]
VILSFRCDETRALFESGSSRRWGNILNVATRKLAMLNAATELRDLRSPPGNRLEPLHGDRAGQHSIRINDQWRICFVWTD
AGPTQVEIVDYH

Nucleotide

• Length: 279 bp

>T28118 NC_018177:c3240703-3240425 [Pseudomonas stutzeri DSM 10701]
GTGATTCTGAGTTTCAGATGCGATGAAACCCGAGCGTTGTTCGAGTCAGGAAGCTCCCGGCGTTGGGGGAACATCCTGAA
CGTCGCGACGAGAAAACTGGCCATGCTCAACGCGGCAACGGAGCTTCGAGACCTGCGCTCTCCACCGGGCAACCGATTGG
AGCCGTTGCATGGCGACCGGGCCGGGCAACATAGCATCAGGATCAATGACCAGTGGCGGATCTGCTTTGTCTGGACTGAC
GCAGGCCCCACACAAGTCGAAATCGTCGACTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11161.64 Da
• Isoelectric Point: 4.8255

>AT28118 WP_014853816.1 NC_018177:c3240412-3240107 [Pseudomonas stutzeri DSM 10701]
MAINGMRPVHPGEVLREEFLEPLGITPAALARALHVSAPTINDIVRERRGVTADTAIRLGRYFDTSAQFWMNLQSDYALA
TTYAANGEEIEHEIEPLRAVG

Nucleotide

• Length: 306 bp

>AT28118 NC_018177:c3240412-3240107 [Pseudomonas stutzeri DSM 10701]
ATGGCAATTAACGGCATGCGCCCCGTTCACCCTGGTGAAGTGTTGCGCGAAGAGTTTCTAGAACCGCTGGGGATTACTCC
CGCTGCGCTTGCTCGTGCGCTGCATGTATCGGCACCGACCATCAACGATATTGTGCGCGAGCGTCGTGGAGTTACTGCTG
ATACGGCTATCCGGCTGGGCCGCTACTTCGATACATCTGCCCAGTTCTGGATGAACCTGCAGTCCGACTACGCATTGGCG
ACGACCTATGCGGCAAACGGCGAAGAGATCGAGCATGAAATAGAGCCGCTTCGGGCGGTTGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1C2E4A9