Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2976596..2977244 Replicon chromosome
Accession NC_018143
Organism Mycobacterium tuberculosis H37Rv

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag RVBD_RS14055 Protein ID WP_003899414.1
Coordinates 2976596..2976919 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag RVBD_RS14060 Protein ID WP_003899415.1
Coordinates 2976999..2977244 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RVBD_RS14035 2972170..2973431 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
RVBD_RS14040 2973805..2975244 - 1440 WP_009935349.1 phage major capsid protein -
RVBD_RS14045 2975252..2975785 - 534 WP_003899412.1 HK97 family phage prohead protease -
RVBD_RS21620 2975938..2976429 - 492 WP_003900541.1 phage terminase small subunit P27 family -
RVBD_RS14055 2976596..2976919 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
RVBD_RS14060 2976999..2977244 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
RVBD_RS14065 2977241..2978668 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
RVBD_RS14070 2978670..2979062 - 393 WP_003899417.1 DUF2742 domain-containing protein -
RVBD_RS14075 2979059..2979319 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
RVBD_RS14080 2979336..2979698 - 363 WP_003900543.1 hypothetical protein -
RVBD_RS14085 2979701..2980828 - 1128 WP_003899420.1 site-specific integrase -
RVBD_RS14090 2980973..2981200 - 228 WP_003899421.1 hypothetical protein -
RVBD_RS21625 2981197..2981586 - 390 WP_003899422.1 hypothetical protein -
RVBD_RS14095 2981492..2981764 + 273 WP_003900544.1 hypothetical protein -
RVBD_RS14100 2981863..2982096 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2970561..2980828 10267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-103)

Antitoxin

Antitoxin MazE-like

(5-68)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T28104 WP_003899414.1 NC_018143:c2976919-2976596 [Mycobacterium tuberculosis H37Rv]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T28104 NC_018143:c2976919-2976596 [Mycobacterium tuberculosis H37Rv]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT28104 WP_003899415.1 NC_018143:c2977244-2976999 [Mycobacterium tuberculosis H37Rv]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT28104 NC_018143:c2977244-2976999 [Mycobacterium tuberculosis H37Rv]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References