Detailed information of TA system TA027930

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 4587674..4588358
• Replicon: chromosome
• Accession: NC_018027
• Organism: Mycobacterium chubuense NBB4

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MYCCH_RS21400
• Protein ID: WP_014817550.1
• Coordinates: 4587674..4588111 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: I4BP76
• Locus tag: MYCCH_RS21405
• Protein ID: WP_014817551.1
• Coordinates: 4588116..4588358 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCCH_RS21370	4583648..4584457	+	810	WP_014817544.1	CbbQ/NirQ/NorQ/GpvN family protein	-
MYCCH_RS21380	4584587..4585285	+	699	WP_041782229.1	hypothetical protein	-
MYCCH_RS21385	4585309..4585722	+	414	WP_014817547.1	hypothetical protein	-
MYCCH_RS21390	4585725..4586945	+	1221	WP_041783374.1	cytochrome P450	-
MYCCH_RS21395	4586990..4587658	+	669	WP_014817549.1	SDR family oxidoreductase	-
MYCCH_RS21400	4587674..4588111	-	438	WP_014817550.1	SRPBCC family protein	Toxin
MYCCH_RS21405	4588116..4588358	-	243	WP_014817551.1	antitoxin	Antitoxin
MYCCH_RS21410	4588369..4590756	-	2388	WP_014817552.1	cation-translocating P-type ATPase	-
MYCCH_RS21415	4590984..4592579	-	1596	WP_014817553.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15708.25 Da
• Isoelectric Point: 10.0651

>T27930 WP_014817550.1 NC_018027:c4588111-4587674 [Mycolicibacterium chubuense NBB4]
MAKLSVSVDVPLPPERAWESASDLSRYKEWLSIHRVWRSKLPDTIEKGTTLDSIVEVKGMPNRVSWTVVHYKPPEAMTLN
GDGKGGVKVKLIGKVKPSELGSTVTFDVHLGGPALFGPIGMVVAGALKGDIQESLNRFKAVFAPS

Nucleotide

• Length: 438 bp

>T27930 NC_018027:c4588111-4587674 [Mycolicibacterium chubuense NBB4]
ATGGCCAAGTTGTCGGTGTCGGTCGACGTGCCGTTGCCCCCGGAAAGGGCATGGGAGAGCGCGTCTGACCTGTCTCGGTA
CAAGGAATGGCTGTCGATTCACCGCGTGTGGCGGTCCAAACTGCCCGACACCATCGAGAAGGGCACGACCCTGGACTCGA
TCGTCGAGGTCAAGGGCATGCCCAACCGGGTGAGCTGGACGGTGGTGCACTACAAGCCGCCGGAGGCGATGACGCTCAAC
GGTGACGGCAAGGGCGGCGTGAAGGTCAAGCTGATCGGCAAGGTCAAGCCCTCAGAACTCGGCTCGACGGTGACCTTCGA
TGTGCACCTGGGCGGCCCCGCGTTGTTCGGGCCGATCGGGATGGTCGTGGCCGGCGCGCTGAAGGGCGACATCCAGGAGT
CGCTGAACCGCTTCAAGGCGGTGTTCGCGCCTTCGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8294.34 Da
• Isoelectric Point: 8.5317

>AT27930 WP_014817551.1 NC_018027:c4588358-4588116 [Mycolicibacterium chubuense NBB4]
MGFLDKAKDLLSKNADKVDTVIDKAGDVVDKKTQGKYASTVDKVQDAAKKAVADNANAANPPAQPPVPPQQQPPAAPPTS

Nucleotide

• Length: 243 bp

>AT27930 NC_018027:c4588358-4588116 [Mycolicibacterium chubuense NBB4]
ATGGGATTTTTGGACAAGGCCAAAGATTTGCTCTCCAAGAACGCCGACAAAGTGGACACGGTGATCGACAAGGCGGGCGA
CGTCGTCGACAAGAAGACCCAGGGCAAGTACGCATCGACGGTCGACAAGGTGCAGGACGCCGCGAAGAAGGCCGTCGCCG
ACAATGCGAACGCCGCCAACCCGCCCGCACAGCCGCCGGTGCCGCCGCAGCAACAGCCCCCGGCTGCTCCGCCCACGTCG
TAA

Structures

Antitoxin

• AlphaFold DB: I4BP76