Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 472826..473463 Replicon chromosome
Accession NC_017912
Organism Bacillus amyloliquefaciens Y2

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag MUS_RS02405 Protein ID WP_003156187.1
Coordinates 473113..473463 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag MUS_RS02400 Protein ID WP_003156188.1
Coordinates 472826..473107 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUS_RS02380 469191..469790 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
MUS_RS02385 469883..470248 + 366 WP_014304402.1 holo-ACP synthase -
MUS_RS02390 470413..471420 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
MUS_RS02395 471537..472706 + 1170 WP_014416966.1 alanine racemase -
MUS_RS02400 472826..473107 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
MUS_RS02405 473113..473463 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
MUS_RS02410 473581..474402 + 822 WP_003156182.1 STAS domain-containing protein -
MUS_RS02415 474407..474772 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
MUS_RS02420 474775..475176 + 402 WP_003156178.1 anti-sigma regulatory factor -
MUS_RS02425 475188..476195 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
MUS_RS02430 476259..476588 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
MUS_RS02435 476585..477067 + 483 WP_007609591.1 anti-sigma B factor RsbW -
MUS_RS02440 477033..477821 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
MUS_RS02445 477821..478423 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T27838 WP_003156187.1 NC_017912:473113-473463 [Bacillus amyloliquefaciens Y2]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T27838 NC_017912:473113-473463 [Bacillus amyloliquefaciens Y2]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT27838 WP_003156188.1 NC_017912:472826-473107 [Bacillus amyloliquefaciens Y2]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT27838 NC_017912:472826-473107 [Bacillus amyloliquefaciens Y2]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References