Detailed information of TA system TA277461

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 93440..93866
• Replicon: plasmid unnamed3
• Accession: NZ_CP122637
• Organism: Escherichia coli strain ETEC4085

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: QDW62_RS27400
• Protein ID: WP_001372321.1
• Coordinates: 93440..93565 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	93642..93866 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QDW62_RS27365 (88463)	88463..88690	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
QDW62_RS27370 (88827)	88827..89498	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
QDW62_RS27375 (89692)	89692..90075	-	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
QDW62_RS27380 (90398)	90398..91000	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
QDW62_RS27385 (91297)	91297..92118	-	822	WP_001234445.1	DUF932 domain-containing protein	-
QDW62_RS27390 (92229)	92229..92525	-	297	WP_001272251.1	hypothetical protein	-
QDW62_RS27395 (92825)	92825..93121	+	297	Protein_113	hypothetical protein	-
QDW62_RS27400 (93440)	93440..93565	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
QDW62_RS27405 (93507)	93507..93656	-	150	Protein_115	plasmid maintenance protein Mok	-
- (93644)	93644..93709	+	66	NuclAT_0	-	-
- (93644)	93644..93709	-	66	NuclAT_1	-	-
QDW62_RS27410 (93678)	93678..93857	+	180	WP_001309233.1	hypothetical protein	-
- (93642)	93642..93866	+	225	NuclAT_0	-	-
- (93642)	93642..93866	-	225	NuclAT_0	-	Antitoxin
- (93642)	93642..93866	-	225	NuclAT_0	-	Antitoxin
- (93642)	93642..93866	-	225	NuclAT_0	-	Antitoxin
- (93642)	93642..93866	-	225	NuclAT_0	-	Antitoxin
QDW62_RS27415 (93835)	93835..94597	-	763	Protein_117	plasmid SOS inhibition protein A	-
QDW62_RS27420 (94594)	94594..95028	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
QDW62_RS27425 (95097)	95097..95684	-	588	Protein_119	hypothetical protein	-
QDW62_RS27430 (95954)	95954..96790	-	837	WP_000480968.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
QDW62_RS27435 (96790)	96790..97593	-	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T277461 WP_001372321.1 NZ_CP122637:c93565-93440 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT277461 NZ_CP122637:c93866-93642 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin