Detailed information of TA system TA027744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 578518..579116
• Replicon: chromosome
• Accession: NC_017856
• Organism: Methylophaga frappieri

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I1YFL9
• Locus tag: Q7C_RS02605
• Protein ID: WP_001896384.1
• Coordinates: 578518..578841 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: Q7C_RS02610
• Protein ID: WP_000058216.1
• Coordinates: 578838..579116 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Q7C_RS02575	574231..574617	-	387	WP_000180225.1	hypothetical protein	-
Q7C_RS02580	574614..575186	-	573	WP_001944091.1	type IV conjugative transfer system lipoprotein TraV	-
Q7C_RS02585	575261..576550	-	1290	WP_014703136.1	TraB/VirB10 family protein	-
Q7C_RS02590	576550..577449	-	900	WP_014703137.1	type-F conjugative transfer system secretin TraK	-
Q7C_RS02595	577433..578059	-	627	WP_000667170.1	hypothetical protein	-
Q7C_RS02600	578056..578337	-	282	WP_000433891.1	type IV conjugative transfer system protein TraL	-
Q7C_RS02605	578518..578841	+	324	WP_001896384.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Q7C_RS02610	578838..579116	+	279	WP_000058216.1	helix-turn-helix domain-containing protein	Antitoxin
Q7C_RS02615	579142..579777	-	636	WP_014703138.1	DUF4400 domain-containing protein	-
Q7C_RS02620	579764..580324	-	561	WP_014703139.1	hypothetical protein	-
Q7C_RS02625	580334..582154	-	1821	WP_000661885.1	conjugative transfer system coupling protein TraD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	376210..618754	242544

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12281.33 Da
• Isoelectric Point: 9.7658

>T27744 WP_001896384.1 NC_017856:578518-578841 [Methylophaga frappieri]
MSWKIDFYDGVEDQILDMPPKIQARMIKLLELMEKHGANLGPPHTESMGDGLFEIRAKAQEGIGRGLFCYLKGKHIYVLH
AFVKKSQKTPKNELNLARDRQKEVQKS

Nucleotide

• Length: 324 bp

>T27744 NC_017856:578518-578841 [Methylophaga frappieri]
ATGAGCTGGAAGATCGACTTTTACGATGGCGTTGAGGATCAGATCCTCGATATGCCTCCCAAAATACAAGCTCGCATGAT
TAAGCTTTTAGAGCTGATGGAAAAGCATGGTGCAAACTTGGGGCCTCCTCATACTGAGTCTATGGGCGATGGGTTATTTG
AAATCCGCGCCAAAGCTCAAGAAGGTATTGGTAGAGGCTTATTTTGCTACTTAAAAGGGAAGCACATTTATGTGTTACAC
GCTTTTGTAAAAAAATCTCAAAAGACGCCCAAAAATGAGCTCAATCTTGCCAGAGATAGACAAAAAGAGGTTCAAAAATC
ATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10517.07 Da
• Isoelectric Point: 9.9393

>AT27744 WP_000058216.1 NC_017856:578838-579116 [Methylophaga frappieri]
MSLQKLKQRALANAEVKNEYDKLESEFSFIDQLLTMRTKAGLTQEQVAERMHTQKSNVSRLEKGNANPSWSTLLKYAHAC
GFELTLKPQKMS

Nucleotide

• Length: 279 bp

>AT27744 NC_017856:578838-579116 [Methylophaga frappieri]
ATGAGTCTGCAAAAACTGAAACAACGTGCTTTAGCCAATGCTGAAGTTAAGAATGAGTACGATAAGCTTGAGAGTGAGTT
CAGCTTTATTGATCAATTGCTTACCATGAGAACAAAGGCAGGACTTACTCAAGAACAAGTTGCTGAAAGAATGCATACCC
AAAAAAGTAATGTCAGTCGTTTAGAAAAAGGTAATGCCAATCCAAGTTGGTCAACTCTTCTAAAATATGCTCACGCATGT
GGCTTTGAACTAACACTCAAGCCACAAAAAATGAGTTAA

Structures

Toxin

• AlphaFold DB: A0A244CN87