Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2683495..2684036 Replicon chromosome
Accession NZ_CP122319
Organism Escherichia coli strain BW25113

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag QCG47_RS12810 Protein ID WP_000615983.1
Coordinates 2683495..2683773 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag QCG47_RS12815 Protein ID WP_000729703.1
Coordinates 2683776..2684036 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QCG47_RS12795 (2681077) 2681077..2681655 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
QCG47_RS12800 (2681861) 2681861..2682628 + 768 WP_000333380.1 class II glutamine amidotransferase -
QCG47_RS12805 (2682599) 2682599..2683339 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
QCG47_RS12810 (2683495) 2683495..2683773 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
QCG47_RS12815 (2683776) 2683776..2684036 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
QCG47_RS12820 (2684246) 2684246..2684995 + 750 WP_000056849.1 C40 family peptidase -
QCG47_RS12825 (2685171) 2685171..2685668 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
QCG47_RS12830 (2685892) 2685892..2687604 - 1713 Protein_2484 flagellar biosynthesis protein FlhA -
QCG47_RS12835 (2687576) 2687576..2688361 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T276762 WP_000615983.1 NZ_CP122319:c2683773-2683495 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT276762 WP_000729703.1 NZ_CP122319:c2684036-2683776 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References