Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2565352..2566079 Replicon chromosome
Accession NZ_CP120633
Organism Escherichia coli strain USVAST219

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag P6O79_RS12820 Protein ID WP_000550189.1
Coordinates 2565765..2566079 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag P6O79_RS12815 Protein ID WP_000560269.1
Coordinates 2565352..2565768 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6O79_RS12805 (2560712) 2560712..2563063 + 2352 WP_001531994.1 alpha-glucosidase -
P6O79_RS12810 (2563289) 2563289..2565307 + 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
P6O79_RS12815 (2565352) 2565352..2565768 - 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
P6O79_RS12820 (2565765) 2565765..2566079 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
P6O79_RS12825 (2566364) 2566364..2567500 - 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
P6O79_RS12830 (2567585) 2567585..2568088 + 504 WP_001295542.1 M48 family metallopeptidase -
P6O79_RS12835 (2568165) 2568165..2568857 + 693 WP_000942551.1 vancomycin high temperature exclusion protein -
P6O79_RS12840 (2568936) 2568936..2569922 + 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T275299 WP_000550189.1 NZ_CP120633:c2566079-2565765 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT275299 WP_000560269.1 NZ_CP120633:c2565768-2565352 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References