Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 2572960..2573596 Replicon chromosome
Accession NZ_CP120608
Organism Priestia megaterium strain DSM 32

Toxin (Protein)


Gene name mazF Uniprot ID D5DWT4
Locus tag P5650_RS15990 Protein ID WP_013055004.1
Coordinates 2573246..2573596 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID D5DWT3
Locus tag P5650_RS15985 Protein ID WP_013055003.1
Coordinates 2572960..2573241 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5650_RS15960 (P5650_15950) 2568326..2569297 + 972 WP_034654859.1 UV DNA damage repair endonuclease UvsE -
P5650_RS15965 (P5650_15955) 2569306..2569908 - 603 WP_034654858.1 rhomboid family intramembrane serine protease -
P5650_RS15970 (P5650_15960) 2569973..2570338 + 366 WP_025753519.1 holo-ACP synthase -
P5650_RS15975 (P5650_15965) 2570397..2571455 + 1059 WP_016762756.1 outer membrane lipoprotein carrier protein LolA -
P5650_RS15980 (P5650_15970) 2571569..2572759 + 1191 WP_034654856.1 alanine racemase -
P5650_RS15985 (P5650_15975) 2572960..2573241 + 282 WP_013055003.1 hypothetical protein Antitoxin
P5650_RS15990 (P5650_15980) 2573246..2573596 + 351 WP_013055004.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
P5650_RS15995 (P5650_15985) 2573756..2574592 + 837 WP_034654854.1 RsbT co-antagonist protein RsbRA -
P5650_RS16000 (P5650_15990) 2574595..2574951 + 357 WP_013055006.1 STAS domain-containing protein -
P5650_RS16005 (P5650_15995) 2574955..2575356 + 402 WP_013055007.1 anti-sigma regulatory factor -
P5650_RS16010 (P5650_16000) 2575370..2576380 + 1011 WP_013055008.1 PP2C family protein-serine/threonine phosphatase -
P5650_RS16015 (P5650_16005) 2576440..2576772 + 333 WP_013055009.1 anti-sigma factor antagonist -
P5650_RS16020 (P5650_16010) 2576769..2577254 + 486 WP_016762758.1 anti-sigma B factor RsbW -
P5650_RS16025 (P5650_16015) 2577220..2578014 + 795 WP_013055011.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12991.96 Da        Isoelectric Point: 4.8781

>T275246 WP_013055004.1 NZ_CP120608:2573246-2573596 [Priestia megaterium]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10691.22 Da        Isoelectric Point: 8.5339

>AT275246 WP_013055003.1 NZ_CP120608:2572960-2573241 [Priestia megaterium]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0B6AMB9

References