Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3043643..3044370 Replicon chromosome
Accession NC_017631
Organism Escherichia coli ABU 83972

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag ECABU_RS15100 Protein ID WP_000547555.1
Coordinates 3044059..3044370 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag ECABU_RS15095 Protein ID WP_000126293.1
Coordinates 3043643..3044062 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECABU_RS15080 3039806..3042058 - 2253 WP_001107659.1 carbamoyltransferase HypF -
ECABU_RS15085 3042211..3042738 - 528 WP_001078782.1 electron transport protein HydN -
ECABU_RS15090 3043123..3043551 + 429 WP_000536064.1 hypothetical protein -
ECABU_RS15095 3043643..3044062 - 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
ECABU_RS15100 3044059..3044370 - 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
ECABU_RS15105 3044533..3045288 - 756 WP_001305268.1 hypothetical protein -
ECABU_RS15110 3045331..3045801 - 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
ECABU_RS15115 3045794..3046204 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
ECABU_RS15120 3046201..3046968 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
ECABU_RS15125 3046968..3047510 - 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
ECABU_RS15130 3047520..3049229 - 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T27269 WP_000547555.1 NC_017631:c3044370-3044059 [Escherichia coli ABU 83972]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T27269 NC_017631:c3044370-3044059 [Escherichia coli ABU 83972]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT27269 WP_000126293.1 NC_017631:c3044062-3043643 [Escherichia coli ABU 83972]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT27269 NC_017631:c3044062-3043643 [Escherichia coli ABU 83972]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCAGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATACCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References