Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 897552..898352 Replicon chromosome
Accession NZ_CP118014
Organism Escherichia coli strain B-766

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag PT280_RS04310 Protein ID WP_000342449.1
Coordinates 897825..898352 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag PT280_RS04305 Protein ID WP_001277108.1
Coordinates 897552..897818 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT280_RS04285 (893210) 893210..893878 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
PT280_RS04290 (893871) 893871..894929 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
PT280_RS04295 (895174) 895174..896028 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
PT280_RS04300 (896299) 896299..897402 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
PT280_RS04305 (897552) 897552..897818 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
PT280_RS04310 (897825) 897825..898352 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
PT280_RS04315 (898349) 898349..898732 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
PT280_RS04320 (899156) 899156..900265 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
PT280_RS04325 (900313) 900313..901239 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
PT280_RS04330 (901236) 901236..902513 + 1278 WP_000803798.1 branched chain amino acid ABC transporter permease LivM -
PT280_RS04335 (902510) 902510..903277 + 768 WP_000082110.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T272673 WP_000342449.1 NZ_CP118014:897825-898352 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT272673 WP_001277108.1 NZ_CP118014:897552-897818 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References