Detailed information of TA system TA027224

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1183048..1183846
• Replicon: chromosome
• Accession: NC_017628
• Organism: Escherichia coli IHE3034

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1RDD2
• Locus tag: ECOK1_RS05855
• Protein ID: WP_001094434.1
• Coordinates: 1183472..1183846 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: ECOK1_RS05850
• Protein ID: WP_001552747.1
• Coordinates: 1183048..1183425 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECOK1_RS05820	1179926..1180747	+	822	WP_001234585.1	DUF945 domain-containing protein	-
ECOK1_RS05825	1180747..1180992	+	246	WP_001164966.1	hypothetical protein	-
ECOK1_RS05830	1181086..1181559	+	474	WP_014639472.1	antirestriction protein	-
ECOK1_RS05835	1181575..1182051	+	477	WP_001186727.1	RadC family protein	-
ECOK1_RS05840	1182114..1182335	+	222	WP_000692315.1	DUF987 domain-containing protein	-
ECOK1_RS05845	1182354..1182998	+	645	WP_000086759.1	hypothetical protein	-
ECOK1_RS05850	1183048..1183425	+	378	WP_001552747.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
ECOK1_RS05855	1183472..1183846	+	375	WP_001094434.1	TA system toxin CbtA family protein	Toxin
ECOK1_RS05860	1183843..1184334	+	492	WP_000976859.1	hypothetical protein	-
ECOK1_RS05865	1184346..1184543	+	198	WP_000839257.1	DUF957 domain-containing protein	-
ECOK1_RS05870	1184636..1185478	+	843	WP_001280460.1	DUF4942 domain-containing protein	-
ECOK1_RS05880	1185949..1186887	+	939	WP_000351298.1	glyoxylate/hydroxypyruvate reductase GhrA	-
ECOK1_RS05885	1186942..1187679	+	738	WP_000283664.1	zinc-binding phosphatase	-
ECOK1_RS05890	1187703..1188257	+	555	WP_001001909.1	molecular chaperone YcdY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13921.82 Da
• Isoelectric Point: 6.8517

>T27224 WP_001094434.1 NC_017628:1183472-1183846 [Escherichia coli IHE3034]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T27224 NC_017628:1183472-1183846 [Escherichia coli IHE3034]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATAACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13757.63 Da
• Isoelectric Point: 6.3127

>AT27224 WP_001552747.1 NC_017628:1183048-1183425 [Escherichia coli IHE3034]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRTGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT27224 NC_017628:1183048-1183425 [Escherichia coli IHE3034]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCACCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGAAGCTGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCACCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: Q1RDD2