Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3604487..3605214 Replicon chromosome
Accession NC_017626
Organism Escherichia coli 042

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag EC042_RS17995 Protein ID WP_000550189.1
Coordinates 3604900..3605214 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID D3GWQ0
Locus tag EC042_RS17990 Protein ID WP_000560253.1
Coordinates 3604487..3604903 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC042_RS17980 3599902..3602253 + 2352 WP_000695464.1 alpha-glucosidase -
EC042_RS17985 3602424..3604442 + 2019 WP_000121429.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
EC042_RS17990 3604487..3604903 - 417 WP_000560253.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
EC042_RS17995 3604900..3605214 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
EC042_RS18005 3605499..3606635 - 1137 WP_000018671.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
EC042_RS18010 3606720..3607223 + 504 WP_001603805.1 M48 family metallopeptidase -
EC042_RS18015 3607300..3607983 + 684 WP_001183061.1 vancomycin high temperature exclusion protein -
EC042_RS18020 3608062..3609048 + 987 WP_024261645.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T27211 WP_000550189.1 NC_017626:c3605214-3604900 [Escherichia coli 042]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T27211 NC_017626:c3605214-3604900 [Escherichia coli 042]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAATCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14965.39 Da        Isoelectric Point: 4.4547

>AT27211 WP_000560253.1 NC_017626:c3604903-3604487 [Escherichia coli 042]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT27211 NC_017626:c3604903-3604487 [Escherichia coli 042]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACGCCCAGGCGCTGGAACTGGTCGATCATCTGCTGCTCAACGATCCTGAAAATCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCTGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATTGGCAGTAAATCCATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure
AlphaFold DB A0A829IXD3

References