Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 914192..914846 Replicon chromosome
Accession NZ_CP117714
Organism Escherichia coli strain PBS4

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag PTA50_RS04500 Protein ID WP_000244777.1
Coordinates 914439..914846 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag PTA50_RS04495 Protein ID WP_000354046.1
Coordinates 914192..914458 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PTA50_RS04470 (909361) 909361..910104 + 744 WP_000951948.1 SDR family oxidoreductase -
PTA50_RS04475 (910161) 910161..911594 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
PTA50_RS04480 (911639) 911639..911950 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
PTA50_RS04485 (912114) 912114..912773 + 660 WP_000250274.1 hemolysin III family protein -
PTA50_RS04490 (912969) 912969..913949 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
PTA50_RS04495 (914192) 914192..914458 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
PTA50_RS04500 (914439) 914439..914846 + 408 WP_000244777.1 protein YgfX Toxin
PTA50_RS04505 (914886) 914886..915407 - 522 WP_001055870.1 flavodoxin FldB -
PTA50_RS04510 (915519) 915519..916415 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
PTA50_RS04515 (916440) 916440..917150 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
PTA50_RS04520 (917156) 917156..918889 + 1734 WP_128995306.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T272081 WP_000244777.1 NZ_CP117714:914439-914846 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT272081 WP_000354046.1 NZ_CP117714:914192-914458 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References