Detailed information of TA system TA027184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 340928..342617
• Replicon: chromosome
• Accession: NC_017626
• Organism: Escherichia coli 042

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: D3GT62
• Locus tag: EC042_RS01655
• Protein ID: WP_000365816.1
• Coordinates: 341697..342617 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: EC042_RS01650
• Protein ID: WP_001134468.1
• Coordinates: 340928..341710 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC042_RS30670	336238..336395	-	158	Protein_307	integrase	-
EC042_RS30675	336487..336589	-	103	Protein_308	helix-turn-helix domain-containing protein	-
EC042_RS01640	336586..337056	-	471	WP_000201185.1	hypothetical protein	-
EC042_RS29930	337102..337242	-	141	WP_001308408.1	ash family protein	-
EC042_RS01645	337403..340612	-	3210	WP_000222103.1	hypothetical protein	-
EC042_RS01650	340928..341710	+	783	WP_001134468.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
EC042_RS01655	341697..342617	+	921	WP_000365816.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
EC042_RS01660	343060..343944	-	885	WP_001119039.1	LysR family transcriptional regulator	-
EC042_RS01665	344209..345267	+	1059	WP_122452191.1	alpha/beta hydrolase	-
EC042_RS01670	345409..346536	+	1128	WP_000171073.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34668.96 Da
• Isoelectric Point: 6.6478

>T27184 WP_000365816.1 NC_017626:341697-342617 [Escherichia coli 042]
MDLRDSYTRQVKLLMDALPHVAKESGFALKGGTAINLFVQDFPRLSVDIDLAYKTFADRDTDLTAINDALKRITASLNSK
PGITAIRQENKTDEKRIIVNTADAQIKIEVSPVWRGLLLPPAEMSVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERITTLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T27184 NC_017626:341697-342617 [Escherichia coli 042]
ATGGATCTGCGTGATAGCTATACCCGGCAGGTCAAACTGTTGATGGACGCTCTGCCGCATGTGGCGAAGGAGTCCGGTTT
CGCATTAAAAGGCGGCACCGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATCGATCTGGCGTATA
AGACTTTTGCGGATCGCGATACCGATTTGACGGCGATCAACGACGCTTTGAAGAGAATTACTGCATCGCTGAACAGTAAA
CCGGGGATTACCGCGATTCGGCAGGAAAATAAAACGGATGAAAAGCGCATCATTGTGAATACGGCTGATGCCCAGATTAA
GATCGAGGTTAGTCCGGTCTGGCGTGGGCTATTGTTGCCTCCGGCAGAAATGTCGGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACTATGAGTGTCGTTTCGCTGGCGGATCTTTATGGCGGTAAAATCTGCGCAGCGCTTGATCGTCAGCAC
CCGCGCGATCTGTTTGATGTGCTGAATATGCTGGAGAAACCGGGACTGATGCGGGAGATCTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGCCCCATTGCCGAGTTGCTGGCTCCAAACTGGGATACAGAGCGGATCACGACGCTGTATGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTGCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATCTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGTAATCTGGCGGTGCTGAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGCGTAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29151.43 Da
• Isoelectric Point: 8.1287

>AT27184 WP_001134468.1 NC_017626:340928-341710 [Escherichia coli 042]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVTSLTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGMNLLSPRKLQALLCASHSVKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPREFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT27184 NC_017626:340928-341710 [Escherichia coli 042]
TTGCCAAACTATCTAAACTGGCTGATGCAGCACACGCTACCCGGCCAGGTGCTGCTCCAGTCATGGCTTACCCGCAACGG
CATAGATCGTAAGCTCTCTTATCTCTATGTGAAGAATGGCTGGCTCAGGCGGATCGCTCACGGCGTATACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTTCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGCCATGCGCACTATACCGAACCGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTTAA
GCTGCCGGGATGGTTTGCTGCCTTCGAACAATCTGCAACATTTATCACGCTGTATACATCGGGGCTTACCGTAGATGTAA
CGTCATTGACTACGGAACTGAAAATTGGCGACCGCCAGTTAGCAGGCAGTGCCCCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATATCGTTCGAACATGCCGATGCTCTGTTTCAGGGGATGAATCTTCTCAGCCCGCGTAAATT
GCAGGCGTTACTCTGCGCCAGTCATTCGGTTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATCTTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCTGGTGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCACGCGAATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• AlphaFold DB: D3GT62