Detailed information of TA system TA027177

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 3933118..3933530
• Replicon: chromosome
• Accession: NC_017625
• Organism: Escherichia coli DH1

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: ECDH1_RS19435
• Protein ID: WP_000132601.1
• Coordinates: 3933189..3933530 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	3933118..3933194 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECDH1_RS19425	3929981..3931570	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
ECDH1_RS19430	3931567..3932961	+	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
-	3933118..3933194	-	77	NuclAT_14	-	Antitoxin
-	3933118..3933194	-	77	NuclAT_14	-	Antitoxin
-	3933118..3933194	-	77	NuclAT_14	-	Antitoxin
-	3933118..3933194	-	77	NuclAT_14	-	Antitoxin
-	3933118..3933194	-	77	NuclAT_15	-	Antitoxin
-	3933118..3933194	-	77	NuclAT_15	-	Antitoxin
-	3933118..3933194	-	77	NuclAT_15	-	Antitoxin
-	3933118..3933194	-	77	NuclAT_15	-	Antitoxin
ECDH1_RS19435	3933189..3933530	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
ECDH1_RS19440	3933692..3935071	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
ECDH1_RS19445	3935071..3936117	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T27177 WP_000132601.1 NC_017625:3933189-3933530 [Escherichia coli DH1]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T27177 NC_017625:3933189-3933530 [Escherichia coli DH1]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT27177 NC_017625:c3933194-3933118 [Escherichia coli DH1]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin