Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1800112..1800943 Replicon chromosome
Accession NC_017625
Organism Escherichia coli DH1

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag ECDH1_RS08745 Protein ID WP_000854814.1
Coordinates 1800112..1800486 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag ECDH1_RS08750 Protein ID WP_001285584.1
Coordinates 1800575..1800943 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECDH1_RS08710 1795508..1796674 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
ECDH1_RS08715 1796793..1797266 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
ECDH1_RS08720 1797464..1798522 + 1059 WP_001200891.1 FUSC family protein -
ECDH1_RS08725 1798694..1799023 + 330 WP_000450409.1 DUF496 family protein -
ECDH1_RS08730 1799124..1799306 - 183 WP_001016348.1 hypothetical protein -
ECDH1_RS23675 1799378..1799506 + 129 Protein_1659 transposase domain-containing protein -
ECDH1_RS25590 1799795..1799908 - 114 WP_001161660.1 DUF957 domain-containing protein -
ECDH1_RS08740 1799921..1800115 - 195 WP_000988600.1 hypothetical protein -
ECDH1_RS08745 1800112..1800486 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
ECDH1_RS08750 1800575..1800943 - 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
ECDH1_RS08755 1801017..1801238 - 222 WP_000692323.1 DUF987 domain-containing protein -
ECDH1_RS25095 1801301..1801747 - 447 WP_000187523.1 RadC family protein -
ECDH1_RS08765 1801744..1803276 - 1533 WP_001350525.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T27161 WP_000854814.1 NC_017625:c1800486-1800112 [Escherichia coli DH1]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T27161 NC_017625:c1800486-1800112 [Escherichia coli DH1]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT27161 WP_001285584.1 NC_017625:c1800943-1800575 [Escherichia coli DH1]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT27161 NC_017625:c1800943-1800575 [Escherichia coli DH1]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References