Detailed information of TA system TA027016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2702403..2702986
• Replicon: chromosome
• Accession: NC_017564
• Organism: Yersinia enterocolitica subsp. palearctica Y11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U7FJS5
• Locus tag: Y11_RS12575
• Protein ID: WP_005162526.1
• Coordinates: 2702403..2702681 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: Y11_RS12580
• Protein ID: WP_005162529.1
• Coordinates: 2702681..2702986 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y11_RS12565	2697884..2698831	-	948	WP_005162520.1	trehalose operon repressor TreR	-
Y11_RS12570	2699603..2702323	+	2721	WP_005162523.1	magnesium-translocating P-type ATPase	-
Y11_RS12575	2702403..2702681	+	279	WP_005162526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Y11_RS12580	2702681..2702986	+	306	WP_005162529.1	HigA family addiction module antidote protein	Antitoxin
Y11_RS12585	2703058..2704986	-	1929	WP_005162532.1	BglG family transcription antiterminator	-
Y11_RS12590	2705059..2706300	-	1242	WP_005162536.1	lactonase family protein	-
Y11_RS12595	2706430..2707170	-	741	Protein_2467	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10694.10 Da
• Isoelectric Point: 7.4011

>T27016 WP_005162526.1 NC_017564:2702403-2702681 [Yersinia enterocolitica subsp. palearctica Y11]
MIKSWKHKGLQRFFETGSAAGINPQHVKRIRDRLRFIDLAETIEDVNVAGYKLHPLQGEREGIWSVTVSGNWRITFEFTA
GDAFILNYEDYH

Nucleotide

• Length: 279 bp

>T27016 NC_017564:2702403-2702681 [Yersinia enterocolitica subsp. palearctica Y11]
ATGATAAAAAGTTGGAAACACAAAGGACTGCAACGCTTCTTTGAAACAGGTTCTGCCGCGGGCATTAATCCCCAGCATGT
TAAGCGAATTCGAGACCGCTTGCGATTTATCGACCTGGCGGAAACGATTGAGGATGTCAATGTTGCCGGATACAAGCTCC
ATCCGTTACAAGGTGAAAGAGAAGGGATCTGGTCAGTAACAGTAAGCGGAAATTGGCGCATTACATTCGAGTTTACCGCT
GGAGATGCGTTTATTCTTAACTACGAGGACTACCACTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10934.77 Da
• Isoelectric Point: 6.7158

>AT27016 WP_005162529.1 NC_017564:2702681-2702986 [Yersinia enterocolitica subsp. palearctica Y11]
MKMHNPPHPGEMIEGILEDLGLGIREFARALNVSPSTVQRLVACKAAISPEMAVKLAAVLGSTPAMWLRLQAAYDLGKAE
ENVDVSNLTKLYKPDPISLHS

Nucleotide

• Length: 306 bp

>AT27016 NC_017564:2702681-2702986 [Yersinia enterocolitica subsp. palearctica Y11]
ATGAAAATGCATAATCCCCCACATCCAGGGGAAATGATTGAAGGAATCCTCGAAGATTTGGGTCTAGGGATAAGGGAGTT
TGCCAGGGCATTAAACGTGTCACCTTCAACGGTACAACGGCTGGTTGCCTGTAAGGCGGCAATTTCACCGGAAATGGCGG
TAAAACTGGCCGCGGTTCTGGGAAGTACCCCAGCAATGTGGCTCCGCCTCCAGGCCGCTTATGACCTGGGAAAAGCAGAA
GAGAATGTTGATGTATCCAATCTGACTAAACTGTACAAACCGGATCCAATATCGCTGCATAGTTGA

Structures

Toxin

• AlphaFold DB: A0A7U7FJS5