Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2963432..2964080 Replicon chromosome
Accession NC_017522
Organism Mycobacterium tuberculosis CCDC5180

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag CCDC5180_RS14045 Protein ID WP_003899414.1
Coordinates 2963432..2963755 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag CCDC5180_RS14050 Protein ID WP_003899415.1
Coordinates 2963835..2964080 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCDC5180_RS14015 2958755..2959753 + 999 WP_003900538.1 tyrosine-type recombinase/integrase -
CCDC5180_RS14020 2959767..2960231 + 465 WP_003900539.1 ParB N-terminal domain-containing protein -
CCDC5180_RS14025 2960219..2960470 + 252 WP_003908028.1 hypothetical protein -
CCDC5180_RS14030 2960641..2962080 - 1440 WP_003901443.1 phage major capsid protein -
CCDC5180_RS14035 2962088..2962621 - 534 WP_003899412.1 HK97 family phage prohead protease -
CCDC5180_RS21660 2962774..2963265 - 492 WP_003900541.1 phage terminase small subunit P27 family -
CCDC5180_RS14045 2963432..2963755 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
CCDC5180_RS14050 2963835..2964080 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
CCDC5180_RS14055 2964077..2965504 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
CCDC5180_RS14060 2965506..2965898 - 393 WP_003899417.1 DUF2742 domain-containing protein -
CCDC5180_RS14065 2965895..2966155 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
CCDC5180_RS14070 2966172..2966534 - 363 WP_003900543.1 hypothetical protein -
CCDC5180_RS14075 2966537..2967664 - 1128 WP_003899420.1 site-specific integrase -
CCDC5180_RS14080 2967809..2968036 - 228 WP_003899421.1 hypothetical protein -
CCDC5180_RS21665 2968033..2968422 - 390 WP_003899422.1 hypothetical protein -
CCDC5180_RS14085 2968328..2968600 + 273 WP_003900544.1 hypothetical protein -
CCDC5180_RS14090 2968699..2968932 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2958755..2967664 8909


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-44)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T26867 WP_003899414.1 NC_017522:c2963755-2963432 [Mycobacterium tuberculosis CCDC5180]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T26867 NC_017522:c2963755-2963432 [Mycobacterium tuberculosis CCDC5180]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT26867 WP_003899415.1 NC_017522:c2964080-2963835 [Mycobacterium tuberculosis CCDC5180]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT26867 NC_017522:c2964080-2963835 [Mycobacterium tuberculosis CCDC5180]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References