Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 210841..212464 Replicon plasmid unnamed1
Accession NZ_CP116348
Organism Enterobacter ludwigii strain CM-TZ4

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag PHA72_RS26605 Protein ID WP_000088044.1
Coordinates 210841..212166 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag PHA72_RS26610 Protein ID WP_012477377.1
Coordinates 212171..212464 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PHA72_RS26575 (PHA72_26570) 206827..207684 - 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
PHA72_RS26580 (PHA72_26575) 207700..208008 - 309 WP_001224686.1 hypothetical protein -
PHA72_RS26585 (PHA72_26580) 208557..208982 - 426 WP_000278471.1 hypothetical protein -
PHA72_RS26590 (PHA72_26585) 209235..210050 - 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
PHA72_RS26595 (PHA72_26590) 210063..210473 - 411 WP_000985909.1 hypothetical protein -
PHA72_RS26600 (PHA72_26595) 210574..210780 - 207 WP_000134171.1 hypothetical protein -
PHA72_RS26605 (PHA72_26600) 210841..212166 - 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
PHA72_RS26610 (PHA72_26605) 212171..212464 - 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
PHA72_RS26615 (PHA72_26610) 212865..214253 - 1389 WP_000083579.1 hypothetical protein -
PHA72_RS26620 (PHA72_26615) 214258..214755 - 498 WP_000340139.1 membrane protein -
PHA72_RS26625 (PHA72_26620) 214958..215614 - 657 WP_000462754.1 trypsin-like serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-9 / aph(3'')-Ib / aph(6)-Id - 1..296144 296144


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T268614 WP_000088044.1 NZ_CP116348:c212166-210841 [Enterobacter ludwigii]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT268614 WP_012477377.1 NZ_CP116348:c212464-212171 [Enterobacter ludwigii]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References