Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 149337..150076 Replicon plasmid unnamed1
Accession NZ_CP116348
Organism Enterobacter ludwigii strain CM-TZ4

Toxin (Protein)


Gene name tacT Uniprot ID G8LQ82
Locus tag PHA72_RS26270 Protein ID WP_014172305.1
Coordinates 149591..150076 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID W7NLL2
Locus tag PHA72_RS26265 Protein ID WP_014172304.1
Coordinates 149337..149603 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PHA72_RS26245 (PHA72_26240) 144887..145657 + 771 WP_045343610.1 TraX family protein -
PHA72_RS26250 (PHA72_26245) 145672..145995 + 324 WP_014172383.1 hypothetical protein -
PHA72_RS26255 (PHA72_26250) 145973..146776 - 804 WP_041163021.1 DUF4225 domain-containing protein -
PHA72_RS26260 (PHA72_26255) 147666..148679 - 1014 WP_014172302.1 plasmid replication initiator RepA -
PHA72_RS26265 (PHA72_26260) 149337..149603 + 267 WP_014172304.1 DUF1778 domain-containing protein Antitoxin
PHA72_RS26270 (PHA72_26265) 149591..150076 + 486 WP_014172305.1 GNAT family N-acetyltransferase Toxin
PHA72_RS26275 (PHA72_26270) 150250..151653 + 1404 WP_000125668.1 ISNCY family transposase -
PHA72_RS26280 (PHA72_26275) 151686..152390 - 705 WP_000130816.1 arsenical resistance protein ArsH -
PHA72_RS26285 (PHA72_26280) 152477..152797 + 321 WP_000941305.1 transcriptional regulator -
PHA72_RS26290 (PHA72_26285) 152843..154132 + 1290 WP_000922628.1 arsenic transporter -
PHA72_RS26295 (PHA72_26290) 154145..154570 + 426 WP_000065802.1 glutaredoxin-dependent arsenate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-9 / aph(3'')-Ib / aph(6)-Id - 1..296144 296144
- flank IS/Tn - - 150250..151653 1403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(75-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17496.15 Da        Isoelectric Point: 9.6039

>T268613 WP_014172305.1 NZ_CP116348:149591-150076 [Enterobacter ludwigii]
VGRVTAPEPLSSSHQMAEFVSGEAVLDDWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHVEATGSLRRNM
PDPIPVIILARLAIDASFRGQGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYLHHGFKASQTQERTLFLKLP
Q

Download         Length: 486 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10049.45 Da        Isoelectric Point: 4.4733

>AT268613 WP_014172304.1 NZ_CP116348:149337-149603 [Enterobacter ludwigii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDAAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G8LQ82

Antitoxin

Source ID Structure
AlphaFold DB W7NLL2

References