Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 285379..285876 Replicon chromosome
Accession NZ_CP116094
Organism Escherichia coli strain DETEC-S560

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag PIC78_RS01310 Protein ID WP_001173343.1
Coordinates 285703..285876 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag PIC78_RS01305 Protein ID WP_000398619.1
Coordinates 285379..285675 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC78_RS01285 (282110) 282110..282829 - 720 WP_000611288.1 two-component system response regulator DcuR -
PIC78_RS01290 (282826) 282826..284457 - 1632 WP_001216477.1 sensor histidine kinase -
PIC78_RS01295 (284638) 284638..284868 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
PIC78_RS01300 (284880) 284880..285152 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
PIC78_RS01305 (285379) 285379..285675 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
PIC78_RS01310 (285703) 285703..285876 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
PIC78_RS01315 (285995) 285995..287512 - 1518 WP_001295074.1 lysine--tRNA ligase -
PIC78_RS01320 (287749) 287749..289206 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T267895 WP_001173343.1 NZ_CP116094:285703-285876 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT267895 WP_000398619.1 NZ_CP116094:285379-285675 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References