Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4017759..4018413 Replicon chromosome
Accession NZ_CP116044
Organism Escherichia coli strain STEC1575

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag PHA55_RS19565 Protein ID WP_000244772.1
Coordinates 4017759..4018166 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag PHA55_RS19570 Protein ID WP_000354046.1
Coordinates 4018147..4018413 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PHA55_RS19545 (4013716) 4013716..4015449 - 1734 WP_271287473.1 single-stranded-DNA-specific exonuclease RecJ -
PHA55_RS19550 (4015455) 4015455..4016165 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
PHA55_RS19555 (4016190) 4016190..4017086 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
PHA55_RS19560 (4017198) 4017198..4017719 + 522 WP_001055874.1 flavodoxin FldB -
PHA55_RS19565 (4017759) 4017759..4018166 - 408 WP_000244772.1 protein YgfX Toxin
PHA55_RS19570 (4018147) 4018147..4018413 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
PHA55_RS19575 (4018656) 4018656..4019636 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
PHA55_RS19580 (4019713) 4019713..4020372 - 660 WP_000250274.1 hemolysin III family protein -
PHA55_RS19585 (4020536) 4020536..4020847 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
PHA55_RS19590 (4020892) 4020892..4022325 + 1434 WP_050864001.1 6-phospho-beta-glucosidase BglA -
PHA55_RS19595 (4022382) 4022382..4023125 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T267718 WP_000244772.1 NZ_CP116044:c4018166-4017759 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT267718 WP_000354046.1 NZ_CP116044:c4018413-4018147 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References