Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4635114..4635924 Replicon chromosome
Accession NZ_CP115831
Organism Klebsiella pneumoniae strain L4-FAA5

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag PGO70_RS22210 Protein ID WP_004178461.1
Coordinates 4635114..4635647 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag PGO70_RS22215 Protein ID WP_002887278.1
Coordinates 4635658..4635924 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PGO70_RS22205 (4633945) 4633945..4635066 + 1122 WP_009309849.1 cupin domain-containing protein -
PGO70_RS22210 (4635114) 4635114..4635647 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
PGO70_RS22215 (4635658) 4635658..4635924 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
PGO70_RS22220 (4636027) 4636027..4637460 - 1434 WP_032446518.1 Cu(+)/Ag(+) sensor histidine kinase -
PGO70_RS22225 (4637450) 4637450..4638133 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
PGO70_RS22230 (4638305) 4638305..4639687 + 1383 WP_032446519.1 efflux transporter outer membrane subunit -
PGO70_RS22235 (4639705) 4639705..4640049 + 345 WP_025987660.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T267408 WP_004178461.1 NZ_CP115831:c4635647-4635114 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT267408 WP_002887278.1 NZ_CP115831:c4635924-4635658 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References