Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4660116..4660926 Replicon chromosome
Accession NZ_CP115701
Organism Klebsiella pneumoniae strain 2020CK-00201

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag PF326_RS23250 Protein ID WP_002887280.1
Coordinates 4660116..4660649 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag PF326_RS23255 Protein ID WP_002887278.1
Coordinates 4660660..4660926 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PF326_RS23245 (PF326_23245) 4658947..4660068 + 1122 WP_002887282.1 cupin domain-containing protein -
PF326_RS23250 (PF326_23250) 4660116..4660649 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
PF326_RS23255 (PF326_23255) 4660660..4660926 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
PF326_RS23260 (PF326_23260) 4661029..4662462 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
PF326_RS23265 (PF326_23265) 4662452..4663135 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
PF326_RS23270 (PF326_23270) 4663307..4664692 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
PF326_RS23275 (PF326_23275) 4664710..4665054 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T267299 WP_002887280.1 NZ_CP115701:c4660649-4660116 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT267299 WP_002887278.1 NZ_CP115701:c4660926-4660660 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References